德氏假单胞菌(Pseudomonas delafieldii)R-8脱硫基因启动子的克隆与鉴定  

Cloning and Characterization of the dsz Promoter Region from Pseudomonas delafieldii R-8

作　　者：余志坚[1,2] 李焕杰[1] 王海胜[1] 李信[1] 

机构地区：[1]中国农业科学院研究生院生物化学与分子生物学实验室,北京100081 [2]东华理工大学生物系,江西抚州344000

出　　处：《江苏农业学报》2009年第3期529-533,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家"863"计划项目(2006AA02Z209)

摘　　要：利用PCR方法,从专一性脱硫菌德氏假单胞菌(Pseudomonas delafieldii)R-8中克隆了脱硫基因启动子系列缩短的片段,连接至启动子探测型表达载体pPR9TT,电转原始菌R-8,并测定重组菌R-8-P中的报告基因LacZ表达量。结果表明脱硫基因核心启动子区缩短至300 bp,并对启动子序列进行了预测。The dsz promoter serial deletion fragments were cloned by PCR from strain Pseudomonas delafieldii R-8 which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate. Series fragments were cloned into the detection of expression vector pPR9TT,then reintroduced into strain R-8 to obtain engineering strains R-8-P, and the expression of reporter gene, LacZ, in R-8-P was determined. The results showed that the size of the dsz core promoter region was 300 bp, and regulation region was forecasted.

关 键 词：德氏假单胞菌 脱硫基因 启动子 

分 类 号：Q785[生物学—分子生物学]