鸭生长素基因单核苷酸多态性分析  

Single Nucleotide Polymorphism Analysis of Duck Ghrelin Gene

作　　者：李慧芳[1] 朱文奇[1] 徐文娟[1] 宋卫涛[1] 高燕[2] 陈宽维[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225003 [2]福建农林大学动物科学学院,福建福州350002

出　　处：《江苏农业学报》2009年第3期576-582,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2008BADB2B08);江苏省高技术研究计划项目(BG2007323);江苏省六大人才高峰项目

摘　　要：根据GenBank上已经公布的鸭生长素(Ghrelin)基因的DNA全序列(GenBank登陆号:EF613552)设计8对引物,通过PCR-SSCP和DNA测序方法寻找高邮鸭、金定鸭、北京鸭、建昌鸭、连城白鸭、攸县麻鸭、绍兴鸭及莆田黑鸭等8个国家级保护鸭品种Ghrelin基因单核苷酸突变位点。结果发现3个突变位点,分别为157 bp处9 bp的缺失、431 bp处T→C的突变、909 bp处A→G的突变(该突变引起了苏氨酸→丙氨酸的变化)。北京鸭的等位基因A频率显著高于其他鸭品种;绍兴鸭的等位基因C频率显著高于其他鸭品种;攸县麻鸭等位基因E频率高于其他鸭品种;连城白鸭的等位基因A、C和E的频率均低于其他品种,反映了连城白鸭独特的种质特性。Based on the complete DNA sequence of duck＇ s Ghrelin gene （GenBank No：EF613552） , 8 primers were designed. The single nucleotide polymorphism （SNP） of Ghrelin gene was investigated in eight national duck breeds, including Gaoyou duck,Jinding duck, Beijing duck, Jianchang duck, Liancheng duck, Yonxian Partridge duck, Shaoxing duck and Putian black duck by PCR-SSCP and DNA sequencing. The results showed that there were 9 bp deletions at the site of 157 bp, T to C mutation at 431 bp site, A to G mutation at 909 bp site which caused Thr to Ala change. The A allele frequency of Beijing duck, C allele frequency of Shaoxing duck and E allele frequency of Youxian Partridge duck was highest in the 8 duck breeds. The frequency of A, C and E allele in Liancheng duck was lower than that in other breeds, respectively, which indicated the special trait of Lianeheng duck breed.

关 键 词：鸭 生长素基因 多态性 PCR—SSCP 

分 类 号：Q754[生物学—分子生物学]