甜椒CMS不育基因ISSR的分子标记  

ISSR Marker of Cytoplasmic Male Sterility in Sweet Pepper

作　　者：刘金兵[1] 王述彬[1] 潘宝贵[1] 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014

出　　处：《江苏农业学报》2009年第3期607-609,共3页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技基础设施建设计划项目(BM2008008);江苏省农业科学院基金项目[6510711;CX(08)610]

摘　　要：运用ISSR技术对甜椒CMST6A及其相应的保持系T6B基因组DNA进行了比较分析,共使用了44条随机引物,其中33条引物在两系之中都获得了扩增产物。在不育系中获得了1条稳定扩增的特异片段ISSR-81400。通过随机抽取群体的不育和可育单株对ISSR引物进行鉴定,初步认为ISSR-81400可能是与甜椒CMS不育基因连锁的分子标记。The comparative analysis of genomic DNAs between sweet pepper cytoplasmic male sterile line CMST6A and its maintainer line T6B was made by means of ISSR technique. Thirty-three primers amplified products in the two lines from 44 random primers, among which the specific fragment ISSR-81400of CMST6A was found. The primer ISSR-8 was identified with sterile plants and fertile plants. The result showed the marker ISSR-81400 is likely linked with male sterile gene.

关 键 词：甜椒 胞质雄性不育 ISSR标记 

分 类 号：S641.303.2[农业科学—蔬菜学]