对氨基水杨酸钠对亚慢性铅暴露大鼠海马超微结构的影响  

Effects of sodium para-aminosalicylic acid on hippocampal ultramicro-structure of subchronic lead-exposed rats

作　　者：邓祥发[1,2] 区仕燕[1] 姜岳明[1] 陈海滨[1] 邓欣[1] 卢珊[1] 王坤[1] 蒋艺华[1] 李罡[1] 陆继培[1] 

机构地区：[1]广西医科大学公共卫生学院卫生毒理学教研室,广西南宁530021 [2]广西医科大学基础医学院人体解剖学教研室

出　　处：《毒理学杂志》2009年第3期213-216,共4页Journal of Toxicology

基　　金：广西科学研究与技术开发计划项目(桂科攻0632007-3F)

摘　　要：目的探讨对氨基水杨酸钠(PAS-Na)对亚慢性铅暴露大鼠海马超微结构的影响。方法雄性大鼠每日腹腔注射(ip)醋酸铅6 mg/kg,每周5 d,连续5周。每日背部皮下注射PAS-Na100或200 mg/kg,连续3周。采用石墨炉原子吸收光谱法测定血铅,透射电子显微镜观察大鼠海马颗粒层超微结构变化。结果铅中毒大鼠血铅含量明显增高,海马超微结构出现病理性改变。PAS-Na 200 mg/kg治疗使铅中毒大鼠海马轴突、树突超微结构恢复接近正常,髓鞘板层结构较紧密,神经原纤维排列紊乱及断裂程度减轻,突触结构比较清晰。结论PAS-Na对铅致大鼠海马神经元的损害可能有拮抗作用。Objective To study the effects of sodium para-aminosalicylic acid （PAS-Na） on hippocampal ultramicro-structure of subchronic lead-exposed rats. Methods Male rats were intraperitoneally given lead acetate （6 mg/kg） daily for 5 weeks （5 days· week）. And then, rats were treated with PAS-Na at 100 or 200 mg/kg by subcutaneous injection in the back for 21 days. The contents of lead in rat blood were measured by graphite furnace atomic absorption spectrometry, and the alterations of ultramicro-structure in hippocampal granular layer of rats were observed by transmission electron microscope. Results The increased contents of lead in blood and the pathological changes of hippocampal uhramicro-structure in the lead-exposed rats were observed. The ultramicro-structures of axon and cytodendrite in hippocampus of the lead-exposed rat treated with PAS-Na（200 mg/kg） were nearly returned to normal level, the structures in layers of medullary sheath were more compact, the levels of derangement and interruption in neurofibril were alleviated, the structures of synapse were more clear than those of lead-exposed rat. Conclusion PAS-Na could be an antagonism on the injured neurons in bippocampus of rats induced by lead.

关 键 词：对氨基水杨酸钠 大鼠 海马 超微结构 

分 类 号：R135.1[医药卫生—劳动卫生]