流动注射-抑制化学发光法测定法莫替丁、西咪替丁和雷尼替丁  

Determination of famotidine,cimetidine and ranitidine by flow injection chemiluminescence suppression method

作　　者：何树华[1] 何德勇[2] 

机构地区：[1]长江师范学院化学系,重庆408003 [2]井岗山学院化学化工学院,井岗山343009

出　　处：《分析试验室》2009年第7期50-53,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委科学研究(KJ051305)项目资助

摘　　要：在弱碱性的多聚磷酸钠介质中，二价铜离子、过氧化氢和染料罗丹明B混合可以产生较强的化学发光，法莫替丁、西咪替丁和雷尼替丁对此发光有较强的抑制作用，其抑制发光强度与法莫替丁、西咪替丁和雷尼替丁的质量浓度在0．005-1．0mg／L范围内呈线性关系，基于此，结合流动注射技术，建立了一种直接测定法莫替丁、西咪替丁和雷尼替丁的化学发光新方法。该方法对法莫替丁、西咪替丁和雷尼替丁的检出限分别为2．7、2．2、2．0μg/L。对500-L法莫替丁、西咪替丁和雷尼替丁平行测定11次，其相对标准偏差分别为2．1％，2．4％，2．5％。该方法已成功用于药品中法莫替丁、西咪替丁和雷尼替丁含量的测定。In sodittm polyphosphate medium, hydroxyl radical could be generated by the reaction of Cu^2＋ with H2O2, and the hydroxyl radical could oxidize rhodamine B to produce CL. Famotidine, cimetidine and ranitidine could decrease the CL intensity. The decreased CL intensities were linear with the concentrations of famotidine, cimetidine and ranitidine in the range of O. 005 - 1.0 mg/L. The new flow injection chemiluminescence （FI-CL） systems for determi- nation of famotidine, cimetidine and ranitidine were established. Their detection limits were 2.7, 2.2 and 2.0μg/L for famotidine, cimetidine and ranitidine, respectively. The relative standard deviations for 500 tLg/L famotidine, cimetidine and ranitidine solution were 2.1%, 2.4% and 2.5% （ n = 11）, respectively. The mechanism of the novel CL reaction was also discussed. The proposed method had been successfttlly applied to the determination of famoticline, cimetidine and ranitidine in pharmaceutical preparation.

关 键 词：抑制化学发光 流动注射分析 法莫替丁 西咪替丁 雷尼替丁 

分 类 号：O557.3[理学—热学与物质分子运动论]