CD44^＋／CD24^-细胞在乳腺癌组织及细胞系中的数量与分布  

Quantity and distribution of CD44^＋/CD24ˉ cells in breast cancer tissue and the cell lines

作　　者：吕新全[1] Zhenhe Suo 马长路[1] 徐珂佳[2] 刘羿杉[2] 李惠翔[1] 

机构地区：[1]广州大学第一附属医院病理科河南省肿瘤病理重点实验室,450052 [2]广州大学第一附属医院肿瘤科,450052 [3]Department of Pathology, the Norwegian Radium Hospital, Rikshospitalet University Hospital, Faculty Division the Norwegian Radium Hospital, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway

出　　处：《中华病理学杂志》2009年第7期441-444,共4页Chinese Journal of Pathology

基　　金：河南省基础与前沿技术研究计划项目（08230045460）

摘　　要：目的检测CD44^＋／CD24ˉ细胞在乳腺癌组织及细胞系中的分布及数量，探讨其与乳腺癌常用标志物表达和乳腺癌分子亚型的关系。方法采用免疫组织化学SP双染及单染法，分别检测了60例乳腺浸润性导管癌中的CD44及CD24的共表达情况和ER、PR、HER2、人雌激素诱导蛋白PS2、bel-2、nm23的单独表达情况，同时检测了三种乳腺癌细胞系（MCF-7、MDA—MB-468及MDA-MB-231）中CD44及CD24的表达情况。结果不同病例标本中CD44^＋／CD24ˉ细胞的数量差异较大，分布无明显规律，总阳性率为65．0％；CD44’／CD24一细胞数量与患者年龄、肿瘤大小、淋巴结转移情况及ER、PR、HER-2、人雌激素诱导蛋白PS2、bel-2、nm23表达情况无关（P均〉0．05）；CD44^＋／CIY24ˉ细胞数量与乳腺癌分子亚型无关。CD44^＋／CD24ˉ细胞在MCF-7、MDA—MB-468及MDA—MB-231细胞系中的比例分别为〈1％、5％及〉80％。结论CD44^＋／CD24ˉ细胞存在于部分乳腺癌组织及细胞系中，其数量及分布与乳腺癌的分子亚型和临床病理参数无直接关系。Objective To study the distribution and quantity of CD44^＋/CD24ˉ cells in breast cancer tissue and the cell lines, and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma. Methods The expression of CD44^＋ / CD24ˉ, estrogen receptor, progesterone receptor, HER2, human estrogen-induced protein PS2, bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining. The co-expression of CD44 and CD24 in 3 breast cancer cell lines （MCF-7, MDA-MB-468, and MDA-MB- 231 ） was also examined. Results The quantity and distribution of CD44^＋/CD24ˉcells varied greatly and no specific patterns were identified. The percentage of CD44 ^＋/CD24ˉ in breast cancer was 65%. The amount of CD44 ^＋/CD24ˉ cells did not correlate with the age of patients, lymph node metastasis, tumor size, molecular subtypes and expression of various breast cancer markers in breast carcinoma. The proportion of CD44^＋/CD24ˉ cells in MCF-7, MDA-MB-468, and MDA-MB-231 cell lines was 〈 1%, 5% and 〉 80% , respectively. Conclusions CD44 ^＋/CD24ˉ cells are demonstrated in certain breast cancer tissues and cell lines. However, there is no relationship obtained between the quantity or the distribution of these ceils and the molecular subtyping or the clinieopathologic parameters in breast cancer.

关 键 词：乳腺肿瘤 免疫表型分型 抗原 CD44 抗原 CD24 肿瘤细胞 培养的 

分 类 号：R686[医药卫生—骨科学]