磁性阿霉素维拉帕米清蛋白纳米粒的制备及性能检测  

Preparation and measurement of magnetic adriamycin-verapamil-albumin nanoparticles

作　　者：王治伟[1] 杨强[1] 赵渝[2] 王蓓蕾[3] 

机构地区：[1]重庆市第二人民医院普通外科,重庆402160 [2]重庆医科大学附属第一医院血管外科,重庆400016 [3]天津南开大学化学系,天津300071

出　　处：《中国普通外科杂志》2009年第6期592-595,共4页China Journal of General Surgery

基　　金：重庆市卫生局2008科研项目(2008-2-346)

摘　　要：目的制备磁性阿霉素维拉帕米清蛋白纳米粒并检测其磁性、载药量、粒径大小等物理性能,为进一步进行动物实验和临床应用奠定基础。方法先将市售棉籽油精制,再将盐酸阿霉素、盐酸维拉帕米、人血清蛋白及Fe3O4磁粉按一定比例混合,采用加热固化法制备磁性阿霉素维拉帕米清蛋白纳米粒。用透射电子显微镜检测颗粒形态和粒径大小。倒置显微镜下观察载药纳米粒的磁响应性和结构。绘制标准曲线,高效液相色谱仪检测纳米粒中维拉帕米的载药量,荧光分光光度仪检测阿霉素的载药量。结果纳米粒大小均一,形态规则,呈圆球形,磁响应性好。平均粒径约0.5μm,维拉帕米载药量为0.8%,阿霉素载药量为1.25%。结论加热固化法可以制备出同时包载两种药物的纳米粒,物理性能良好,制备方法简便,实验条件容易控制。Objective To prepare magnetic adriamycin-verapamil-albumin nanoparticles （MAVANs） and measure the physical property of the MAVANs, such as magnetism, drug loading （DL）and size of nanoparticles in order to direct its further experimental study on animals and its application in clinical practice. Methods Commercial cottonseed oil was refined, then mixed with adriamycin, verapamil, human albumin and Fe3O4 magnetic powder according to appropriate proportion, and MAVANs was prepared by using a heat denaturation technique. The size and shape of nanoparticles were determined by electron transmission micro-photography. The magnetic response and structure of the MAVANs were analyzed by upside-down microscope. The standard curve was plotted. The drug loading of verapamil in the MAVANs was quantified by high performance lignid chromatography. The drug loading of adriamycin was quantified by a standard speetrophotonuorometry. Results The nanoparticles featured homogenous size, regular and spherical shape, and good magnetic response. The mean diameter of nanoparticles was 0. 5 μm. The loading amount of verapamil was 0. 8 %. The drug loading of adriamycin was 1. 25 %. Conclusions The heat denaturation technique is feasible to prepare the nanoparticles which can load two kinds of drug with good physical property. The process is simple and the testing condition is easy to control.

关 键 词：磁性纳米粒 阿霉素 维拉帕米 清蛋白 

分 类 号：R358.3[医药卫生—基础医学]