猪源和鼠源脑心肌炎病毒分离株基因组的比较分析  

Comparative Analysis of the Genomes of Encephalomyocarditis Virus Isolates from Porcine and Mouse Origin

作　　者：赵婷[1] 张家龙[1] 盖新娜[1] 郭鑫[1] 周磊[1] 陈艳红[1] 查振林[1] 杨汉春[1] 

机构地区：[1]中国农业大学动物医学院,农业部人畜共患病重点开放实验室,北京100193

出　　处：《畜牧兽医学报》2009年第6期873-878,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金重点项目(30530550);教育部长江学者和创新团队发展计划(IRT0866)

摘　　要：为了分析猪源和鼠源脑心肌炎病毒（EMCV）基因组的差异,对分离自同一猪场猪临床样本和鼠组织样本的2株病毒（GX0601和GX0602）进行了全基因组序列测定和比较分析。结果显示,2个分离毒株的基因组全长（包含poly A）分别为7 729和7 725 nt,核苷酸和氨基酸同源性均在99.8%以上。与国外毒株及国内已报道的分离株的序列比较结果表明,2个毒株与BJC3和HB1的核苷酸同源性为98.18%~99.41%,推导的氨基酸序列同源性为99.5%~99.7%,与国外分离株的核苷酸序列和氨基酸序列的同源性分别介于80.53%~99.57%和93.1%~99.5%。基于基因组开放阅读框推导的氨基酸序列的系统进化分析表明,我国分离毒株与国外毒株PEC9、CBNU、BEL-2887A/91和EMCV-R的同源性较高,位于同一个亚群。研究结果提示,猪源与鼠源毒株的基因组具有很高的同源性,鼠是猪场EMCV感染的传染来源。The complete genomes of two strains of encephalomyocarditis virus （EMCV） （GX0601 and GX0602）, isolated from clinical tissue samples of pig and mouse in a pig farm in Guangxi province, were sequenced and analyzed in order to investigate the differences between the ge- nomes of viruses from different host origin. The results showed that the genome sizes of the two viruses were 7 729 and 7 725 nt, respectively, including poly （A） tail. The complete genomic nu- cleotide and amino acid identity between the two viruses were above 99.8%. Compared with for- eign and domestic strains, the two viruses had 98.18%-99.41% identity in nucleotide and 99.5%-99.7% identity in deduced amino acid with BJC3 and HB1, while 80.53%-99.57% identi- ty in nucleotide and 93.1%-99.5% identity in deduced amino acid with foreign strains. Phyloge- netic tree based on the amino acid sequences of the entire ORF revealed that the Chinese isolates clustered together with PECg, CBNU,BEL-2887A/91 and EMCV-R. Our results revealed that the complete genome between the viruses from pig and mouse origin had higher homology, sug- gesting that mouse might be the source of EMCV infection in pig farm.

关 键 词：脑心肌炎病毒 猪源和鼠源毒株 基因组 序列测定 进化分析 

分 类 号：S852.659.6[农业科学—基础兽医学]