绿色荧光蛋白标记大肠杆菌气溶胶的发生及其传播模式  

Occurence and Transmission of Airborne Escherichia coli Marked by Green Fluorescent Protein

作　　者：刘凤芝[1] 柴同杰[1] 王海荣[1] 李晓霞[1] 苗增民[1] 王伟[1] 袁文[1] 张红双[1] 秦梅[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2009年第6期886-891,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571381);山东省自然科学基金(Y2006D14)

摘　　要：为了认识微生物气溶胶的发生与传播规律,作者建立了细菌气溶胶传播模型,即试验鸡摄入用绿色荧光蛋白(GFP)基因标记的E.coliJ M109后,检测这种大肠杆菌在鸡体的排出及其形成气溶胶与传播过程。鸡饮完含E.coliJ M109-pGFP菌液(5.0×1010CFU.只-1)后,采用ANDERSEN-6级空气微生物样品收集器分别在鸡栏内、栏外2、5、10、20 m收集气载E.coliJ M109-pGFP。通过每一个采样点J M109-pGFP浓度的变化来评估动物舍微生物气溶胶向舍外环境的传播情形。结果显示,试验鸡饮完含E.coliJ M109-pGFP的饮水,0.5 h左右开始排出含E.coliJ M109-pGFP的粪便,此时测量栏内和栏外2、5、10、20 m处J M109-pGFP气溶胶浓度达最高,分别为1.531×103、8.080×102、3.360×102、2.250×102、6.600×10 CFU.m-3;栏内与栏外不同距离间的J M109-pGFP浓度差异不显著(P>0.05)。30 h时只能在栏框内收集到J M109-pGFP,为7 CFU.m-3;36 h后在栏框内也消失。结果表明,动物排泄物中的细菌能够形成气溶胶,并能通过舍内外气体交换传播到舍外,造成周边环境的生物污染。In order to study the occurence and transmission of airborne microorganism,a model to estimate spread of the airborne microorganism was established in this study by giving chicken with culture medium contain E. coli JM109 strain marked with green fluorescent protein. Air samples, including pen and outside of the pen air （2 and 5 m as well as 10, 20 m away） of chicken house were collected using six-stage Andersen microbial samplers. E. coli JM109-pGFP concen- trations （CFU· m-3 air） collected from different sampling sites were calculated. Through the E. coli JM109-pGFP obtained from different sampling sites the spreading of bioaerosol from animal houses to the ambient air was characterized. After drinking E. coli JM109-pGFP culture medium half an hour,chicken excreted feces containing E. coli JM109-pGFP,at the same time the isolated E. coli JM10g-pGFP concentrations in pen air and outside sites of the pen （2 and 5 m as well as 10, 20 m away） were reached top concentrations, were 1. 531 × 10^3, 8. 080 × 10^2, 3. 360 × 10^2, 2. 250× 10^2, 6. 600 × 10 CFU ·m-3 ,but there were no significant difference between the inside and the outside sites of the pen （P〉O. 05）. After 30 h, E. coli JM109-pGFP concentration was 7CFU · m-3 , E. coli JM109-pGFP wasn＇t detected in other sites. After 36 h,E. coli JM109-pG- FP disappeared in pen air. Our results suggested that E. coli JM109-pGFP isolated from pen air and outside of the pen air originated in the chicken feces and the microbiological aerosols in the chicken house were transmitted by air exchanging to the surroundings, so it could cause environmental microbiological pollution to some extents.

关 键 词：绿色荧光蛋白 大肠杆菌气溶胶 试验鸡舍 气溶胶传播模式 

分 类 号：S851.2[农业科学—预防兽医学] R181[农业科学—兽医学]