δ差异显示法筛选仔猪ETEC F4ad受体相关基因  

Screening Genes Related to Piglet ETEC F4ad Receptor with δDDRT-PCR Method

作　　者：蒋隽[1] 施启顺[1] 刘鑫[1] 马海明[1] 贺长青[1] 柳小春[1] 

机构地区：[1]湖南农业大学动物科技学院,长沙410128

出　　处：《畜牧兽医学报》2009年第6期939-943,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目资助(30371030)

摘　　要：初生仔猪泻痢的病原菌主要是肠毒素大肠杆菌(ETEC)F4,仔猪小肠上皮细胞有无F4受体对于仔猪抗病性具有重要意义。本试验采用δ差异显示法筛选仔猪F4ad受体相关基因。将获得的8条ESTs通过GenBank进行序列同源比对,发现其中1条与人糖基磷脂酰肌醇锚1(GPI1)基因有91%同源性。表明该基因可作为F4ad受体候选基因进行进一步研究。Enterotoxigenic Escherichia coli （ETEC） F4 was one of the most serious pathogenic bacteria inducing piglet diarrhea, ETEC F4 receptor in small intestine epithelium cells is of great importance for piglet＇s disease-resistance breeding. Differential display reverse transcription （DDRT） PCR method was used to screen genes related to piglet ETEC F4 receptor in this study, 8 ESTs were compared with GenBank. The result showed that one of them had 91% homologous to human glycosylphosphatidylinositol anchlor 1（GPI 1）, which indicated that the GPI 1 gene might be the candidate gene of F4ad receptor.

关 键 词：肠毒素大肠杆菌 F4ad受体 δ差异显示 糖基磷脂酰肌醇锚1 仔猪 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]