寻常型银屑病患者外周血单个核细胞TLR9mRNA的表达及与IFN-γ的相关性分析  

Investigation on the Expression of TLR9 and Its correlation with IFN-γ in Patient with Psoriasis Vulgaris

作　　者：罗权[1] 林玲[1] 刘玉梅[1] 朱慧兰[1] 何玉清[1] 周欣[1] 曾抗[2] 张锡宝[1] 

机构地区：[1]广东省广州市皮肤病防治所,广东广州510095 [2]南方医科大学南方医院皮肤科,广东广州510515

出　　处：《岭南皮肤性病科杂志》2009年第3期152-154,157,共4页Southern China Journal of Dermato-Venereology

基　　金：广东省自然科学基金(项目编号:07001961);广州市医药卫生科研重点项目(项目编号:2006-ZDi-07)

摘　　要：目的:探讨寻常型银屑病患者外周血外周血单一核细胞(PBMCs)Toll样受体9(Toll-like receptor9,TLR9)的表达及其与血清中IFN-γ浓度的相关性,初步探讨TLR9在寻常型银屑病发病中的作用。方法:采用荧光定量PCR技术检测外周血单个核细胞TLR9的表达,并采用双抗体夹心酶联免疫吸附法(ELISA)检测血清IFN-γ的浓度。另取20例正常人作为对照组。结果:寻常型银屑病患者外周血中单核细胞TLR9的表达较正常人对照组明显增高(P<0.01),TLR9表达与血清IFN-γ浓度变化呈正相关(P<0.05)。结论:结果提示TLR9及其介导的天然免疫应答在银屑病发病机制中起重要作用,其机制可能是通过上调IFN-γ的表达促使炎症因子产生和分泌而介导银屑病的发病。Objective：To explore the expression of TLR9 in human peripheral blood mononuclear cell and its correlation with concentration of IFN - γ in patient with psoriasis vulgaris. Methods：The TLR9mRNA in peripheral blood mononuclear cell were measured by Real -time quantitative PCR. The study group was composed of 30 cases of patient with psoriasis vulgaris and 20 normal persons as control. The level of IFN - γ in sera were measured by double antibody sandwich enzyme- linked immunosorbent assay （ELISA）. Results：The expression of TLR9 in human peripheral blood mononuclear cell and the serum level of IFN - γ were significant increased in patients with psoriasis vulgaris as compared with normal control （ P 〈 0.01 ） , and the expression of TLR9 was positively correlated with the concentration of IFN - γ. Conclusion ： The TLR9 and the innate immune response might play a critical role in the pathogenesis of psoriasis vulgaris, which may be through spurring the excretion of inflammatory factor.

关 键 词：银屑病 TOLL样受体 干扰素Γ 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]