硫酸镁对烟草感染PVY^N后病情及防御酶系活性的影响  

Effect of MgSO4 on Disease and Defensive Enzymes of Tobacco after PVY^N Infection

作　　者：顾晶晶[1] 李鑫[1] 赵秀香[1] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161

出　　处：《沈阳农业大学学报》2009年第3期286-290,共5页Journal of Shenyang Agricultural University

基　　金：辽宁省科技攻关项目(2006215004)

摘　　要：对叶面喷施240mg·L-1硫酸镁溶液后,烟草幼苗感染烟草马铃薯Y病毒脉坏死株系(Potato Virus Y-vein necrosis,PVYN)的发病情况、病毒含量及防御酶系活性进行研究。结果表明:烟草幼苗经240 mg·L-1 MgSO4处理,再接种PVYN后其发病程度减轻,病情指数及幼苗叶片中病毒含量均低于未喷施MgSO4的对照。叶面单独喷施MgSO4处理和喷施后接种PVYN处理,对苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)的活性均有较强的促进作用,烟草幼苗接种PVYN后,PAL在处理第6天时达到酶活高峰(2.63U·g-1FW),POD、PPO的活性在接种PVYN后第15天时产生高于对照处理的酶活高峰,峰值分别为55.76U·g-1FW和10.62U·g-1FW;但喷施MgSO4对过氧化氢酶(CAT)活性影响较小。本研究证实,MgSO4具有较强的诱导植物产生抗病毒的能力,可明显提高植株的抗病性。Foliar spray method was introduced to study the effects of MgS04 with the concentration of 240 mg·L^-1 on disease situation and defensive enzymes of tobacco seedlings after PVY^N infection. The results showed that the disease index and the eontent of PVY^N of tobacco seedlings treated with MgSO4 were all lower than that of the inoculation control. MgSO4 had a strong stimulative function on the activities of phenylalanine ammonia-lyase （PAL）, peroxidase （POD） and polyphenol oxidase （PPO） when spraying on leaves. PAL achieved the peak of enzymatic activity on the 6th day after spraying MgSO4（2.63U·g^-1FW）,POD （55.76U·g^-1FW）and PPO （10.62U·g^-1FW）reached the peak on the 15th day, which were higher than that of the control. But MgSO4had a weak influence on eatalase （CAT） activity. This research confirmed that MgSO4 has stronger ability of inducing plants to resist virus and can significantly improve plant＇s disease resistance.

关 键 词：烟草 马铃薯Y病毒脉坏死株系(PVYN) 苯丙氨酸解氨酶 过氧化物酶 多酚氧化酶 过氧化氢酶 MgSO4 

分 类 号：S572[农业科学—烟草工业]