前列腺素E2对阻塞性黄疸大鼠小肠粘膜形态的保护作用  

Protection of PGE2 against intestinal mucosa injuries in obstructed jaundice rats

作　　者：祝林[1] 胡三元[1] 张光永[1] 丁祥就[1] 

机构地区：[1]山东大学齐鲁医院普外科,济南250012

出　　处：《山东大学学报（医学版）》2009年第6期12-14,19,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨前列腺素E2(PGE2)对阻塞性黄疸大鼠小肠粘膜的保护作用。方法Wistar大鼠50只,随机分成阻塞性黄疸组(A组)10只、阻塞性黄疸再通组(B组)10只、阻塞性黄疸PGE2干预组(C组)10只、阻塞性黄疸再通加PGE2干预组(D组)10只和假手术组(E组)10只。采用直接结扎法制成阻塞性黄疸大鼠模型。再通组采用导管法再通。结扎后1周给予PGE2,结扎后2周再通,再通手术后1周取标本,观察大鼠小肠粘膜的形态学变化。结果PGE2干预组小肠粘膜损伤评分为(2.21±0.75),手术再通组大鼠小肠损伤评分为(2.05±0.71),均较黄疸组减轻(5.26±0.57,P<0.05)。结论阻塞性黄疸大鼠小肠粘膜明显受损,PGE2及手术再通均可减少其损伤。但两者没有协同作用。Objective To explore the effect of PGE2 on damage of intestinal mucosa in obstructed jaundice rats. Methods A total of 50 Wistar rats were randomly divided into the obstructive jaundice group （A group, n = 10）, the obstructive jaundice recanalization group （B group, n = 10）, the obstructive jaundice PGE2 intervention group （C group, n = 10）, the obstructive jaundice recanalization and PGE2 intervention group （D group, n = 10） and the sham-operation group （E group, n = 10）. Obstructive jaundice models were established by direct ligation. The re-canalization group was used repeffusion catheterization. PGE2 was used one week after ligation, and materials were drawn at 1 week after reperfusion for observation of morphological changes in the rat small intestine. Results Scores in small intestinal injury of the PGE2 intervention group （2.21 ± 0.75） and scores in small intestine injury of the reeanalization group （2.05 ± 0.71 ）, were less than the obstructive jaundice group （5.26 ± 0.57, P 〈 0.05）. Conclusion Small intestinal mucosa of obstructive jaundice rats was markedly impaired. PGE2 and surgical re-canalization may reduce the injury but they have no synergic effect.

关 键 词：黄疸 阻塞性 前列腺素 小肠 大鼠 WISTAR 

分 类 号：R656[医药卫生—外科学] R-332[医药卫生—临床医学]