人脐带间充质干细胞的无动物血清培养及向肝细胞诱导分化  

Culture of human cord mesenchymal stem cells using animal serum-free medium and hepatogenic differentiation

作　　者：张辉[1] 时庆[2] 侯怀水[2] 李栋[2] 

机构地区：[1]中国农村技术开发中心,北京100045 [2]山东大学齐鲁医院低温医学研究室,济南250012

出　　处：《山东大学学报（医学版）》2009年第6期88-91,94,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Q2005C03和Y2005C27);国家自然科学基金项目(30700163)资助

摘　　要：目的探索从脐带组织中分离培养间充质干细胞的无动物血清方法,并将其向肝细胞诱导分化。方法分离自体脐血清用于后续细胞培养,利用酶消化法从脐带组织中分离培养间充质干细胞,检测其形态和免疫表型,并采用两步法诱导其向肝细胞诱导分化。结果流式细胞术检测结果表明,自体脐血清培养得到的贴壁细胞表型符合间充质干细胞特征,且在合适诱导条件下能肝样细胞分化,经过4周处理,能表达肝细胞特异性基因,部分细胞具有吞噬低密度脂蛋白的能力。结论采用自体脐血清有效地培养脐带间充质干细胞,具备向肝细胞的分化能力。Objective To investigate the protocols of isolating mesenchymal stem cells from the cord using an animal serum-free method and to evaluate their differentiation ability to hepatocytes. Methods Autologous cord blood sertun was used to culture cord mesenchymal stem ceils. An enzyme digesting method was used to isolate mesenchymal stem cells from the cord. Morphology., immunotyping and hepatogenic differentiation of these cells were assayed. Results FACS experiments showed that immunotyping of adherent cells cultured using autologous cord blood serum matched with the characters of mesenchymal stem cells and could differentiate into hepatocytes under the appropriate induction condition. After treatment for 4 weeks, differentiated cells could express hepatocyte-specific markers. Some hepatocyte-like cells could uptake lipoprotein. Conclusion The autologous cord blood serum method could effectively culture mesenchymal stem cells with hepatogenic differentiation ability.

关 键 词：脐带 干细胞 脐血清 细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]