三氯化铁及络合铁的抗银屑病作用研究  

Effect of FeCl_3 and Fe-EDTA on inhibiting psoriasis

作　　者：杨晓倩[1] 季静[1] 刘娜[1] 郭冬梅[1] 崔晞[1] 

机构地区：[1]山东大学公共卫生学院卫生检验研究所,济南250012

出　　处：《山东大学学报（医学版）》2009年第6期114-117,共4页Journal of Shandong University:Health Sciences

基　　金：山东省交通厅资助课题(200424Y22)

摘　　要：目的探讨三氯化铁(FeCl3)及络合铁(Fe-EDTA)抗银屑病的作用。方法建立昆明种雌激素周期雌鼠阴道上皮细胞有丝分裂和雄鼠尾鳞片表皮颗粒层两种银屑病的动物实验模型。将昆明小鼠随机分为阴性对照组、阳性对照组,FeCl3低、中、高剂量组,Fe-EDTA低、中、高剂量组和朴雪口服液组。最后1次给药后,脱颈椎处死小鼠,光学显微镜下观察雌鼠阴道上皮细胞有丝分裂情况及雄鼠尾鳞片表皮颗粒层形成情况,计算有丝分裂指数和100个鳞片中有颗粒层的鳞片数。结果与阴性对照组比较,各补铁组和阳性对照组均可抑制雌鼠阴道上皮细胞的有丝分裂(P<0.05),均对雄鼠尾鳞片表皮颗粒层形成起促进作用(P<0.05);除阴性对照组外,其余各组两两比较,差异均无统计学意义(P>0.05)。结论三氯化铁及络合铁均有一定的抗银屑病作用。Objective To explore the effect of FeCl3 and Fe-EDTA on inhibiting psoriasis. Methods Female Kunming mice were used to establish the epidermis hyperplasia of estrone periodical mouse models, and were randomly divided into nine groups： the negative control, positive control, low, middle, high dosage of FeCl3, low, middle, high dosage of Fe-EDTA and Oral Buxue groups. Male Kunming mice serving as the epidermis parakeratosis models were also randomly divided into the above nine groups. The mice were sacrificed by cervical dislocation after the last administration, and then the index of vaginal mitosis（female mice） and formation of the tail scale epidermis（ male mice） were observed under an optical microscope. Results Every iron spiked groups as well as the methotrexate group could obviously inhibit mitosis of the vaginal epithelial ceils （P 〈 0. 05） and promote formation of the granular layer in mouse tail scale （ P 〈 0.05）. Statistical significance was found between the negative contml and iron spiked group （ P 〈 0.05）, but not among the iron spiked groups （ P 〉 0.05）. Conclusion As a result of iron replenishment, mitosis of the vaginal epithelial cells was obviously inhibited, and formation of the granular layer in mouse tail scale was significantly promoted.

关 键 词：三氯化铁 络合铁 银屑病 小鼠 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]