山东省人类肠道致细胞病变孤儿病毒13型分离株的基因特征分析  

The Genetic Characterization of Enteric Cytopathic Human Orphanviruses Type 13 Isolated in Shandong Province

作　　者：毛乃颖[1] 王海岩[2] 朱贞[1] 李岩[2] 纪峰[2] 陶泽新[2] 崔爱利[1] 许文波[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,病毒基因工程国家重点实验室,世界卫生组织西太平洋区麻疹参比实验室,北京100050 [2]山东省疾病预防控制中心,济南250014

出　　处：《中国疫苗和免疫》2009年第3期238-241,共4页Chinese Journal of Vaccines and Immunization

基　　金：世界卫生组织西太平洋区脊髓灰质炎参比实验室经费;卫生部疾病预防控制专项

摘　　要：目的阐明山东省从急性弛缓性麻痹病例粪便标本中分离到人类肠道致细胞病变孤儿病毒13型(Entric Cytopathic Human Orphanviruses Type13,ECHO13)的VP1基因特征。方法通过逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)扩增ECHO13VP1基因序列,进行PCR产物序列测定和分析,将获得的基因序列与基因库中已有的39株ECHO13 VP1基因序列进行同源性分析,并共同构建基因进化关系树。结果6株ECHO13山东省分离株与之前从福建省分离的ECHO13存在明显的基因差异,最高达20%,并构成了独立的进化分支B组;同时与其他国家分离到的ECHO13也存在明显的基因差异,在进化树上分属不同的分支,说明中国存在不同基因特征的ECHO13传播。结论根据VP1基因的差异,ECHO13可被划分为A、B、C三个组。应加强对肠道病毒的监测和分子流行病学研究,防止国外ECHO13的输入。Objective To analysis the genetic characteristics of Echo 13 isolated from stool specimens of AFP patient in Shandong. Methods The complete VP1 sequences of the 6 isolates were successfully amplified by RT-PCR and were compared with other Echovirus 13 isolates available from GenBank. Results 6 Shandong isolates were clearly identified as Echo 13 and were grouped into B cluster that agreed with neutralization result. The homogeneity analysis showed that the Shandong isolates share at least 80% similarity in nucleotide level and 94.4% in amino acid level. Phylogenetic tree base on complete VP1 sequence indicated there were many transmitting chains of Echovirus 13 prevailed in China. Conclusion All Echo13 isolates were divided into three genomic clusters A, B and C. It is suggested that enterovirus surveillance should be strengthened to prevent imported of Echo 13.

关 键 词：人类肠道致细胞病变孤儿病毒13型 分子流行病学分析 

分 类 号：R373.2[医药卫生—病原生物学]