HPLC法检查注射用盐酸罂粟碱中的有关物质  

Determination of Related Substances in Papaverine Hydrochloride for Injection by HPLC

作　　者：于新颖[1] 吴雨川[1] 刘天扬[1] 

机构地区：[1]黑龙江省药品检验所,哈尔滨150001

出　　处：《黑龙江医药》2009年第4期431-432,共2页Heilongjiang Medicine journal

摘　　要：目的:建立高效液相色谱法检查注射用盐酸罂粟碱中有关物质的方法。方法:采用Dismonsil(Dikma)C18(20cm*4.6mm,5μl)色谱柱,甲醇-0.1%磷酸二氢钠溶液(60:40)为流动相,流速为1.0ml/min,检测波长为250nm,柱温:30℃。结果:各有关物质与主峰能够达到有效分离,检测限为1.912ng/ml。结论:本方法简便、稳定、专属性强,可用于注射用盐酸罂粟碱有关物质的检查。Objective：To establish an HPLC method for determination of related substances in Papaverine Hydrechloride for Injection. Methods ：The Dismonsil （Dikma） C,s （20cm ＊ 4. 6mm, 5ul ）column was used, the mobile phase consisted of methanol - 0. 1% NaH2 PO4 （60：40）, the flow rate was 1.0ml/min, the detection wavelength was 2.50nm, Temperature： 30℃. Results：The peak of related substances vs Papaverine Hydrochloride for Injection can be separated effectively and the limit of detection was 1. 912ng/mL Conclusious：The method is convenient, stable, specific that it can be used to determine the related substances of Papaverine Hydrochloride for Injection.

关 键 词：注射用盐酸罂粟碱 高效液相色谱法 有关物质 

分 类 号：R927.1[医药卫生—药学]