Rhodobacter sphaeroides和Rhodovulum sulidophilum LHII的α和β亚基的交叉作用关系  

Relations on Cross-combination of β-and α-polypeptides of Light Harvesting Complexes Ⅱ (LHII)Between Rhodobacter sphaeroides and Rhodovulum sulidophilum

作　　者：陈国平[1] 李尽哲[1] 胡宗利[1] 王万能[1,2] 潘宇[1] 

机构地区：[1]重庆大学生物工程学院,中国重庆400044 [2]重庆工学院化学与生物工程学院,中国重庆400030

出　　处：《生命科学研究》2009年第3期215-220,225,共7页Life Science Research

基　　金：国家科技部"863"计划项目(2006AA02Z138);重庆市科委自然科学基金项目(CSTC;2007BB1198;2007BB0250)

摘　　要：类球红细菌(Rhodobacter sphaeroides)和嗜硫小红卵菌(Rhodovulum sulidophilum)为不同属的两种光合细菌,前者的捕光系统Ⅱ由pucB、pucA基因编码产生的β亚基和α亚基组装形成,后者的捕光系统Ⅱ由pucsB、pucsA基因编码产生的β亚基和α亚基组装形成.将这两组基因交叉组合,克隆到包含puc启动子的表达载体中,得到两个表达质粒即pRKpucsBpucA和pRKpucBpucsA,然后通过接合转移方法分别转入LHI、LHII和RC缺陷型菌株DD13中,两种接合转移菌株都可以形成捕光系统Ⅱ并进入光合细菌膜系统.Rhodobacter sphaeroides and Rhodovulum sulidophilum belong to different generas of photosynthetic bacteria. β- and α-polypeptides of light harvesting complexes II （LHII）in Rhodobacter sphaeroides and Rhodovulum sulidophilum are encoded respectively by two group of homologous genes, pucB, pucA and pucsB, pucsA. The two group of genes were connected crosswise to form two new permutations, pucB-pucsA and pucsB-pucA, then cloned into the expression vector controlled by puc promoter coming from Rhodobacter sphaeroides, respectively, to yield two plasmids, PRKpucApucsB and PRKpucsApucB, which were introduced into LH I^-, LHII^- and RC^- mutation Rhodobacter sphaeroides （DD13）. The result showed that both of the two transconjugant strains can synthesize LHII complexes and construct into membrane system subsequently.

关 键 词：嗜硫小红卵菌(Rhodovulum sulidophilum) 类球红细菌(Rhodobacter sphaeroides) 捕光系统Ⅱ 交叉组合 

分 类 号：Q55[生物学—生物化学] TP311[自动化与计算机技术—计算机软件与理论]