基于SPR传感的空间相位调制蛋白质芯片检测方法  

Spatial phase modulation protein chip detection based on SPR sensing

作　　者：余兴龙[1] 闫硕[1] 定翔[1] 刘芳芳[1] 

机构地区：[1]清华大学精密仪器与机械学系精密测试技术及仪器国家重点实验室

出　　处：《仪器仪表学报》2009年第6期1134-1139,共6页Chinese Journal of Scientific Instrument

基　　金：国家自然科学基金(30727001);高等学校博士学科点专项科研基金(200500030056)资助项目

摘　　要：无标记蛋白质芯片检测可成为蛋白质组学研究的重要技术平台。将空间相位调制与SPR传感结合,使发生SPR时引起的反射光的相位变化转化为干涉条纹的位置变化,通过分析干涉条纹的位置变化即可获得反射光的相位变化,从而解析出相关生物信息。在构建了空间调制SPR相位检测实验装置的基础上,进行了生理盐水浓度测定以及兔IgG和羊抗兔IgG相互作用检测实验。实验结果表明,NaCl溶液分辨率至少为3×10-5RIU,检测的动态范围达到1.33～1.37,兔IgG与羊抗兔IgG相互作用时引起相位变化12°。这些实验结果表明,该方法适合蛋白质微芯片的检测。Label-free protein detection can be an important technical platform of proteomics research. This paper presents a method combining spatial phase modulation with SPR sensing. The phase change of reflected light can be detected by analyzing the displacement of interference stripes and the interrelated information of biomolecular interaction can be obtained. NaCl solutions at different concentrations are tested and the interaction between rabbit IgG and goat-anti-rabbit IgG is detected. Experiment results demonstrate that the resolution of NaC1 solution can be enhanced to 3×10^-5 RIU at least, the detection dynamic range can reach to 1.33 - 1.37, and the phase change caused by rabbit IgG-goat-anti-rabbit IgG interaction is 12°. These results suggest that this method is fit for the protein chip detection.

关 键 词：表面等离子体共振 空间相位调制 相位检测 蛋白质芯片 蛋白质组学 

分 类 号：TH7[机械工程—仪器科学与技术]