早熟大白菜游离小孢子胚诱导及植株再生的研究  

Studies on embryoid induction of isolated microspore and plantlet formation in precocious Chinese cabbage

作　　者：张亚丽[1] 张鲁刚[1] 张华敏[1] 张立志[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100

出　　处：《甘肃农业大学学报》2009年第3期45-49,共5页Journal of Gansu Agricultural University

基　　金：国家科技支撑计划(2006BAD01A7);农业部公益性行业科研专项(nyhyzx07-007);陕西省重大科技专项(2007ZDKG-05)

摘　　要：为了优化大白菜游离小孢子培养技术,研究了影响大白菜游离小孢子胚状体诱导及植株再生的主要因素,结果表明:不同基因型的小孢子胚状体的发生频率有明显差异,试验的10个基因型中有7个基因型诱导出了胚,其中以早熟大白菜‘陕秋白’产胚量最高,达到每蕾54.8个胚,耐抽薹的‘05BRF1-20’、‘05BRF1-22’和晚熟的‘秦白3号’没有诱导出胚.研究发现植株的生理状态与小孢子的发育关系密切,盛花期取样的小孢子出胚率最高,分别是初花期和末花期的4.65和15.4倍.在NLN-13培养基中附加0.2 mg.L-16-BA能促进胚状体的发生.B5培养基中附加0.1 mg.L-1GA3是小孢子胚芽分化的最佳培养基.1/2MS培养基中附加0.1 mg.L-1NAA为小孢子植株的最适生根培养基.The major factors influencing the formation of embryoid and plantlet in Chinese cabbage microspore culture were investigated. It was found that the rate of embryogenesis was mainly depended on the genotypes. Among the 10 genotypes, 7 produced microspore-derived. The most ideal material for em- bryoid induction was ＇ Shanqiubai＇, its yield was 20＇, ＇05BRF1-22＇ and late-ripen ＇Qinbai 3＇ did 54. 8 embryoids per bud. Late-bolting material ＇05BRF1- not induce embryoids. It was also found a close relationship between plant physiological condition and microspore development. The highest rate of embryogenesis from microspore was the bud sampled in peak flowering period, which is 4. 65 and 15.4 times of begin and end of florescence. The medium of NLN-13 added with 0. 2 mg·L^-1 6-BA promoted the development of embryoids. B5 medium added with 0. 1 mg·L^-1 GA3 was most suitable for microspore plumule differentiation. 1/2 MS medium added with 0. 1 mg·L^-1NAA was most suitable for microspore plant rooting.

关 键 词：大白菜 游离小孢子培养 胚状体 植株再生 

分 类 号：S634.1[农业科学—蔬菜学]