祛湿化瘀方防治实验性脂肪肝的药效学研究  

Pharmacodynamic effect of Qushi Huayu decoction on experimental fatty liver disease in rats

作　　者：顾宏图[1] 李红山 冯琴[1] 许丽莉[1] 彭景华[1] 张宁[1] 胡义扬[1] 

机构地区：[1]上海中医药大学曙光医院.上海中医药大学肝病研究所,上海201203 [2]宁波市传染病医院

出　　处：《中西医结合肝病杂志》2009年第3期154-155,164,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然基金项目(No.30672635);上海市优秀学科带头人计划项目(No.06XD14018)

摘　　要：目的:探讨中药祛湿化瘀方防治脂肪肝的作用。方法:采用Wistar雄性大鼠,以四氯化碳(CCl4)皮下注射复合高脂低蛋白饮食制备大鼠脂肪肝模型。在造模2周后,将造模大鼠随机分为模型组、祛湿化瘀方高剂量组、中剂量组和低剂量组,灌胃用药2周。观察大鼠体重、肝指数、血清丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)、谷氨酰转肽酶(GGT)活性、肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量以及肝组织病理变化(HE染色)。结果:模型组肝组织出现明显大泡样脂肪变性;肝组织TG、FFA含量以及血清ALT、AST、GGT活性显著升高。祛湿化瘀方各剂量组的病理改变较模型组显著减轻;肝组织TG、FFA含量和血清ALT、AST、GGT活性显著低于模型组。其中高剂量组降低ALT的作用显著强于中、低剂量组。结论:祛湿化瘀方防治实验性脂肪肝疗效显著。Objective： To observe the prevention and treatment effects of a traditional Chinese medicinal prescription, Qushi Huayu decoction （QHD）, on fatty liver disease. Methods： The experimental fatty liver disease in male Wistar rats were induced with carbon tetrachloride （ CCl4 ） subcutaneous injection compound with high-fat and low-proteindiet for four weeks. At the beginning of the third week, rats were divided into model, QHD high-dose, QHD middle-dose and QHD low-dose groups and administrated with corresponding medicinal decoction for two weeks via gastrogavage. The body weight, liver index, serum alanine aminotransferase （ ALT）, aspartate aminotransferase （ AST）, gamma-glutamyhranspeptide （ GGT）, hepatic triglyceride （TG）, hepatic free fatty acid （FFA） and the histological changes in liver （H. E. staining and oil red staining） were evaluated. Results ： The obvious hepatic macrosteatosis, increased hepatic TG, FFA, serum ALT, AST and GGT were observ.ed in model group. On the contrast, the histological changes, hepatic TG, FFA, serum ALT, AST and GGT were all meliorated significantly in the QHD high, middle and low-dose groups. Furthermore, the serum ALT activity in the QHD high-dose group was significantly lower than that in the other two QHD groups. Conclusion： QHD can significantly prevent and treat the experimental fatty liver disease.

关 键 词：脂肪肝 肝损伤 甘油三酯 游离脂肪酸 祛湿化瘀方/药效学 

分 类 号：R285[医药卫生—中药学]