无锡地区流感病毒病原学监测及H3亚型血凝素基因变异研究  

Etiology monitoring of influenza virus and variation in hemagglutinin genes of H3 subtype in Wuxi area

作　　者：尤凤兴[1] 居丽雯[2] 马广源[1] 张敬平[1] 施强[2] 吉杏生[1] 吴家林[1] 凌霞[1] 肖勇[1] 沙丹[1] 吴庆刚[1] 季亚勇[1] 

机构地区：[1]无锡市疾病预防控制中心,214023 [2]上海复旦大学公共卫生院

出　　处：《中华实验和临床病毒学杂志》2009年第3期171-173,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：国家卫生部资助项目（WKJ2006-2-10）

摘　　要：目的监测无锡地区2005年至2008年季节性流感型与亚型分布并了解2008年部分A／H3N2分离株血凝素（HA）基因变异状况。方法无锡地区医院门诊流感样患者及集体单位聚集性流感样暴发患者采集鼻咽拭标本，接种MDCK细胞，采用标准抗血清鉴定阳性分离株型与亚型，并对2008年部分H3亚型流感病毒进行HA全基因测序，分析HA基因变异状况。结果2005年至2008年9月，在无锡地区流感样患者中共分离到435株流感病毒，其中164株为A／H1N1亚型，80株为A／H3N2亚型，B型191株。型与亚型有明显季节性分布强弱特征。H3亚型HA序列分析，无锡地区9株分离株与上海地区同期分离株接近，有很多序列相互穿插归属于进化树的同一分支，与WHO2008--2009年疫苗推荐株相近。结论近年无锡地区散发和局部暴发流感病毒感染仍主要为A／H1N1、A／H3N2和B型，A／H3N2HA基因与上海地区同期分离株接近，与WHO2008--2009年疫苗推荐株相近。Objective To monitor the seasonal distribution of influenza types and subtypes in Wuxi area during 2005--2008, and to investigate the variation in hemagglutinin （HA） genes of A/H3N2 strains in 2008. Methods Nose-throat swab specimens were collected in Wuxi area from flu-like patients from outpatient departments of hospitals as well as from clustering flu-like outbreak patients from workspace, followed by MDCK cell inoculation. Types and subtypes of positive influenza isolates were identified using standard antiserum. We then sequenced the HA genes for H3 subtype influenza viruses isolated from 2008 specimens to investigate the variation in HA genes. Results During 2005 and September 2008, 435 strains of influenza viruses were isolated from flu-like patients in Wuxi Area, among which 164 isolates are of A/H1N1 subtype, 80 isolates are of A/H3N2 subtype, and 191 isolates are of B type. These types/subtypes have significant seasonal distributions. Sequences of HA genes for H3 subtype show that the 9 strains isolated in Wuxi area are similar to those of strains isolated in Shanghai within the same period. Many of the sequences belong to the same branch of the phylogenetic tree, and are similar to sequences of vaccine strains in WHO 2008-2009 repositories. Conclusion A/H1N1, A/H3N2 and B still attribute to most of the sporadic and Inca/outbreaks of influenza infection in Wuxi area in recent years. HA genes of A/H3N2 strains isolated in Wuxi area are similar to those of strains isolated in Shanghai in the same period, and also similar to those of vaccine strains recommended by WHO for 2008--2009.

关 键 词：正粘病毒科 监测 免疫学 血凝素类 基因 变异(遗传学) 

分 类 号：R181.3[医药卫生—流行病学]