rhGM—CSF作为成人乙肝疫苗无（弱）应答者免疫佐剂初探  

Study on rhGM-CSF as adjuvant in revaccination among adults of non-and hyporesponders to hepatitis B vaccine

作　　者：陈河香[1] 王向阳[1] 王舜钦[1] 何洪涛[1] 罗小光[1] 陈小刚[1] 林潮双[2] 

机构地区：[1]广东国际旅行卫生保健中心,广州510635 [2]中山大学附属第三医院感染病科

出　　处：《中华实验和临床病毒学杂志》2009年第3期177-179,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：国家质检总局广东出入境检验检疫局科技计划项目（2005GDK44）

摘　　要：目的探讨重组人粒细胞．巨噬细胞集落刺激因子（rhGM—CSF）对提高成人乙肝疫苗无（弱）应答免疫的作用。方法将两年内完成1～2个标准乙肝疫苗接种程序、复查HBV标志物均阴性的健康人群随机分为A、B两组。A组：33人，按标准免疫程序予10μg乙肝疫苗；B组：34人，先予rhGM—CSF300ktg皮下注射，次13始按A组方案予乙肝疫苗。接种首针后第1、2、8个月（T1、12、T8）采血检测抗．HBs。结果A、B组1、8抗-HBs阳性率分别为39．39％和64．71％（P=0．038）；A组三次抗体滴度检测结果无显著性变化；B组升高明显，分别为（113．85±198．56）mIU／ml，（312．40±349．44）mIU／ml，（427．74±411，58）mIU／ml（P=0.001）。A组和B组1、8抗体水平差异有统计学意义（P=0．010）。结论rhGM—CSF联合乙肝疫苗复种对无（弱）应答者免疫效果优于单纯复种，GM—CSF具有提高机体对乙肝疫苗免疫应答的能力。Objective To investigate the effect of recombinant human granulocyte macrophage colony stimulating factor （rhGM-CSF） as adjuvant on immune response in adults of non-and hyporesponders to hepatitis B vaccine. Methods Those who were once immunized with recombined yeast gene hepatitis B vaccine more than one standard scheme in two years and negative for hepatitis B markers were randomly sorted as group A and group B. 33 adults of group A were given hepatitis B vaccine 10 μg each time. The immune procedure was 0, 1 and 6 month. 34 adults of group B were given rhGM-CSF 300 μg for the first day, then 10 μg each time for routine immune. The blood samples were collected before the first injection and in 1, 2 and 8 months （T1, T2, T8 ） following the first injection to test Anti-HBs. Results Anti-HBs positive conversion rates of group A and B at T8 was 39.39% and 64.71% respectively（ P = 0.038）. Anti-HBs levels of group B at T1, T2, T8 were（113.85 ± 198.56）mIU/ml, （312.40 ± 349.44） mIU/ml, （427.74 ± 411.58） mIU/ml（ P = 0.001 ）. There was significant difference between group A and B in T8 Anti-HBs levels（ P = 0.010）. Conclusion Better immune response was found in the group of rhGM-CSF with hepatitis B vaccine. So rhGM-CSF can induce the immune respond to hepatitis B vaccine.

关 键 词：粒细胞-巨噬细胞集落刺激因子 肝炎疫苗 乙型 佐剂 免疫 免疫减弱宿主 

分 类 号：R392[医药卫生—免疫学]