大鼠心室肌细胞调节性细胞容积减小的离子通道机制  

Ion channel mechanism of regulatory volume decrease in rat ventricular myocytes

作　　者：张知非[1] 刘杰[1] 保肇实[1] 史路平[1] 徐萌[1] 王军[1] 

机构地区：[1]首都医科大学基础医学院生理学教研室,北京100069

出　　处：《心脏杂志》2009年第4期445-448,共4页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(30670765);北京市自然科学基金项目资助(7072010)

摘　　要：目的观察成年大鼠心室肌细胞调节性细胞容积减小(regulatory volume decrease,RVD)的过程,探讨参与RVD过程的离子通道机制。方法将急性分离的大鼠心室肌细胞放入低渗溶液中,利用细胞体积测量系统测定细胞平均容积的变化过程和离子通道的参与过程。结果大鼠心室肌细胞具有良好的RVD功能;该过程可被氯通道阻断剂如蒽-9-羧酸(anthracene-9-carboxylic acid,9-AC,500μmol/L)和钾通道阻断剂CsCl(5mmol/L)所阻断。进一步的研究发现,ATP敏感钾通道(KATP)的阻断剂格列苯脲(glibenclamide,10μmol/L)可以明显地抑制细胞的RVD过程。结论成年大鼠心室肌细胞具有RVD功能,RVD过程的完成有赖于氯通道和钾通道的平行激活,ATP敏感钾通道是参与容积调节的钾通道之一。AIM： To observe the regulatory volume decrease （RVD） process of rat ventricular myocytes and to investigate its ion channel mechanism. METHODS： Isolated rat ventricular myocytes were exposed to hypotonic solution and the cell volume was measured using Coulter Counter System. RESULTS： Rat ventricular myocytes showed a RVD process and this process was blocked by Cl^- channel blocker 9-AC and K^＋ channel blocker CsCl. Further results demonstrated that a subtype of the K^＋ channel involved in RVD was an ATP-sensitive K^＋ channel because of its high sensitivity to glibenclamide. CONCLUSION： The RVD process of rat ventricular myocytes is dependent on the parallel activation of Cl^- channel and K^＋ channel. The subtype of K^＋ channel involved in the RVD process is ATP-sensitive K^＋ channel.

关 键 词：调节性细胞容积减小 心室肌细胞 离子通道 大鼠 ATP敏感性钾通道 

分 类 号：R331[医药卫生—人体生理学]