染料介导的光氧化处理去细胞猪主动脉瓣的细胞毒性研究  

Cytotoxicity of acellular porcine heart valve treated with dye-mediated photooxidation

作　　者：刘洋[1] 孙国成[1] 刘珊珊[2] 郑奇军[1] 顾春虎[1] 易定华[1] 刘维永[1] 

机构地区：[1]第四军医大学西京医院心脏外科,陕西西安710032 [2]第四军医大学唐都医院超声诊断科,陕西西安710038

出　　处：《心脏杂志》2009年第4期496-499,共4页Chinese Heart Journal

基　　金：国家高技术研究发展计划(863计划)课题项目资助(2006AA02A138);陕西省科技攻关课题项目资助[2007K11-03(1)]

摘　　要：目的评价染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣膜的细胞毒性。方法将L929细胞经亚甲基蓝介导光氧化处理去细胞猪主动脉瓣浸提液培养后,用MTT比色法测定其相对增殖率。将L929细胞与染料介导光氧化处理去细胞猪主动脉瓣直接接触培养,于不同时间观察细胞的生长状态。将L929细胞种植于染料介导的光氧化处理去细胞猪主动脉瓣表面,用扫描电镜观察其生长情况。结果染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的性质稳定,细胞毒性程度为0～Ⅰ级。L929细胞与瓣膜材料直接接触培养生长良好,形态学无明显改变。结论染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的细胞相容性良好。AIM： To evaluate the cytotoxicity of acellular porcine heart valve treated with dye-mediated photooxidation. METHODS： L929 cells were cultured in the extraction obtained from acellular porcine heart valve with dye-mediated photooxidation to measure the relative growth rate by methyl-thiazolyltetrazolium （MTT） assay. L929 cells were cultured directly with acellular porcine heart valve with dyemediated photooxidation. Cell morphology was observed and the cell number was counted. Morphology of L929 cells cultured on the surface of acellular porcine heart valve with dye-mediated photooxidation was examined-by scanning electron microscopy. RESULTS： The property of acellular porcine heart valve with dye-mediated photooxidation was stable. Toxic levels ranged from grade 0 to Ⅰ . There were no signs of cytotoxicity induced by acellular porcine heart valve with dye-mediated photooxidation, and cell morphology was normal. CONCLUSION： Acellular porcine heart valve with dye-mediated photooxidation shows good cytocompatibility in vitro.

关 键 词：染料介导光氧化 去细胞 生物瓣膜 组织工程 细胞毒性试验 

分 类 号：R318.11[医药卫生—生物医学工程]