灰斑病菌入侵对不同抗性玉米品种保护酶活性的影响  

The Effect of Invasion by the Gray Leaf Spot Pathogen on Protective Enzymes Activity in Different Corn Resistant Varieties

作　　者：王桂清[1] 赵培宝[1] 杜学林[1] 邢光耀[1] 

机构地区：[1]聊城大学农学院,山东聊城252059

出　　处：《华北农学报》2009年第3期149-153,共5页Acta Agriculturae Boreali-Sinica

基　　金：山东省中青年科学家科研奖励基金(2006BS06015);山东省教育厅科学技术项目(J06N01)

摘　　要：为从分子生物学角度进一步揭示玉米对灰斑病抗性的生理机制提供理论依据,利用紫外可见分光光度计,采用比色(酶活测定)法,研究了玉米灰斑病菌侵入对不同抗性玉米品种保护酶活性的影响。结果表明,玉米灰斑病菌侵入后不同抗性玉米品种体内的保护酶活性均发生一定的变化:POD活性增加、CAT活性降低,酶活增加(或降低)的幅度和提高(或降低)率与寄主的抗感性关系不密切;EST活性提高,抗病品种酶活增加的幅度和提高率比感病品种高;SOD活性增加,且感病品种SOD酶活增加的幅度比抗病品种高;PPO活性增加,感病品种酶活提高率较高,而中抗和高抗病品种酶活提高率较低。说明玉米在遭到病菌侵入时,体内会产生相应的反应来减轻危害。It indicated that when corn was hurt, the body would develop appropriate response to mitigate damage. Using the method of chromometry by ultraviolet-visible spectrophotometer, the effect on the activity of protective enzymes in different resistant maize varieties infected by the gray leaf spot pathogens were analyzed. The results showed that the activity of some protective enzymes changed, including：the activity of POD was increased, the activity of CAT was decreased, both changes of the two enzyme activity were not related with the host resistance ; the activity of EST was increased, furthermore both the increase rate and extent of the enzyme activity in disease-resistant varieties were higher than that in susceptible varieties;the activity of SOD was increased, the increase rate and extent of the activity in disease-susceptible varieties were higher than that in resistant varieties; the activity of PPO was increased, the elevation rate of the PPO activity in susceptible varieties were higher,rather than in middling and high disease-resistant varieties.

关 键 词：玉米品种 玉米灰斑病菌 保护酶 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]