赣江赣州段水体有机提取物的雌激素活性观察  

Survey on the estrogen activity in organic extracts of water body from the Ganzhou reach of Ganjiang River

作　　者：胡恭华[1] 李锋[1] 李思思[1] 熊跃玲[1] 黄海燕[2] 庄志雄[2] 

机构地区：[1]赣南医学院预防医学系,江西赣州341000 [2]深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国工业医学杂志》2009年第3期184-187,共4页Chinese Journal of Industrial Medicine

基　　金：江西省教育厅科学技术研究项目(No.赣教技字[2007]302号)

摘　　要：目的了解赣江水体环境内分泌干扰物的污染状况,从而为赣江水体污染的防治提供科学的依据。方法分别于丰水期及枯水期从赣江赣州段的上游、中游和下游各3个断面的水体采集水样,采用XAD-2大孔树脂对水样中的有机物进行浓缩提取,然后利用人乳腺癌MCF-7细胞增殖实验检测有机提取物的雌激素活性。结果各水样有机提取物均显示出雌激素活性,其中上游高于下游,江边高于江心,丰水期高于枯水期。结论赣江水体已受到环境内分泌干扰物的污染,制定相应的防治对策已刻不容缓。Objective To comprehend the pollution status by endocrine disrupting chemicals in Ganjiang River, thereby provide a scientific basis for the prevention and control of the pollution in Ganzhou River. Methods Water samples were collected at three sites located at the upstream, midstream and downstream from the Ganzhou reach of Ganjiang River in high-and low-water season respectively, the organic substances were extracted, separated and condensed by macroporous resin XAD-2, then measure the activity of estrogen using cell proliferation assay in MCF-7 cells. Results The estrogen were found in all the water samples from the Ganzhou reach of Ganjing River, the activity was higher in the midstream and upstream than in the downstream, which were higher in river side and high-water season than that in the river center and low-water season, respectively. Conclusions The results suggested that the water body in Ganjiang River has been polluted by endocrine disrupting chemicals, and it should be quite urgent for making a corresponding prevention and control strategy.

关 键 词：环境内分泌干扰物 MCF-7细胞增殖实验 赣江 

分 类 号：R123.1[医药卫生—环境卫生学]