Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus(Bge.) Hsiao  

蒙古黄芪的组织培养及辐射诱变研究(英文)

作　　者：赵秀娟[1] 张红霞[1] 

机构地区：[1]内蒙古科技大学生物与化学工程学院

出　　处：《Agricultural Science & Technology》2009年第2期37-40,共4页农业科学与技术（英文版）

基　　金：内蒙古科技大学校内基金项目(KJ200611)~~

摘　　要：[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A. membranaceus Bge. [ Method ] With the different parts of Astragalus membranaceus Bge. var. mongholicus （ Bge. ） Hsiao aseptic seedling as explants （ leaves, cotyledons, hypocotyls） induced callus, and cotyledon and hypocotyls taken by the method of radiation mutation were studied. [ Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS ＋6-BA 2.0 mg/L ＋ NAA2.0 mg/L, LS ＋6-BA2.0 mg/L ＋NAA0.1 mg/L, MS ＋ 6-BA2.0 rng/L ＋ NAA2.0 rag/L; the optimum mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. [ Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A. membranaceus Bge.1材料与方法1．1材料供试材料为内蒙古包头市美岱召协力气村采收的蒙古黄芪的种子，蒙古黄芪无菌苗的叶片、子叶和下胚轴。试剂：0．1％升汞溶液、MS培养基、1mol／LNaOH、1mol／LI-ICI、琼脂、蔗糖、2．0mg／ml6-BA、2．0mg／ml NAA、0．1mg／ml NAA、LS培养基。

关 键 词：Astragalus membranaceus Bge. var. mongholicus （ Bge. ） Hsiao Tissue culture Radiation mutation 

分 类 号：S567.239[农业科学—中草药栽培]