转化生长因子β在分泌性中耳炎动物模型鼓室黏膜上皮中的表达及意义  

Expression and Significance of Transforming Growth Factor β_1,and β_2 in the Middle Ear Mucosa of Guinea Pigs with Secretory Otitis Media

作　　者：袁琨[1] 张帆[1] 喻慧岭[1] 贺海霞[1] 向芳[1] 郑婕[1] 

机构地区：[1]武汉市中心医院耳鼻咽喉科,武汉430014

出　　处：《华中科技大学学报（医学版）》2009年第3期364-367,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的检测转化生长因子β1、β2(transforming growth factor β1、β2,TGF-β1、TGF-β2)在分泌性中耳炎(secretory otitis media,SOM)动物模型鼓室黏膜中的表达,探讨TGF-β1、TGF-β2在SOM发病机制中的作用及意义。方法应用灭活肺炎链球菌注入豚鼠中耳腔,制作SOM动物模型,采用免疫组织化学方法检测致病鼠鼓室黏膜上皮在注入细菌后6h、1d、3d、7d、14d及30d后TGF-β1、TGF-β2的表达。结果SOM动物模型的鼓室黏膜上皮中TGF-β1、TGF-β2早期就有表达。TGF-β1在术后7d表达明显增强,第30天时达高峰,TGF-β2在术后24h即有表达,14d时达高峰。结论灭活肺炎链球菌致SOM中,TGF-β1、TGF-β2在鼓室黏膜中出现过度表达,TGF-β1可能与SOM慢性迁延有关,TGF-β2在SOM早期起主导作用。Objective To explore the expression of transforming growth factor β1 , and β2 （TGF-β1 , TGF-β2） in the mucosa of guinea pigs with secretory＇otitis media （SOM） and the role of TGF-β1, and TGF-β2 in the development of SOM. Methods SOM was produced by injecting deactivated streptococcus pneumoniae into the typmpanic cavity of guinea pigs. The expression of TGF-β1, and TGF-β2 was examined by using immunohistochemistry at the 6th hour, 1st day, 3rd day, 7th day, 14th day and 30th day after injection. Results The TGF-β1 , and TGF-β2 expression could be early detected in the middle ear mucosa. TGF-β2 could be detected 24 h after injection and reached the peak at the 14th day. The expression of TGF-β1 began to increase at the 7th day after injection and reached the peak at the 30th day. Conclusion TGF-β1, and TGF-β2 are expressed in the acute stage of SOM induced by deactivated streptococcus pneumoniae, indicating that TGF-β1 may play a role in the chronic protraction of SOM and TGF-β2 may be a important role in the early stage of SOM.

关 键 词：转化生长因子Β 分泌性中耳炎 鼓室黏膜 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]