中药大青叶有效单体抗柯萨奇病毒作用  

Antiviral Activity of the Effective Monomers from Folium Isatidis against Coxackievirus B_3 Virus in Vitro

作　　者：刘钊[1] 杨占秋[2] 肖红[2] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]武汉大学医学院病毒学研究所,武汉430071

出　　处：《中南民族大学学报（自然科学版）》2009年第2期41-45,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学自然科学基金资助项目(YZQ05011)

摘　　要：采用中药大青叶有效单体进行体外抗柯萨奇病毒B3(CVB3)的研究,通过观察病毒引起的细胞病变效应(CPE)、MTT法检测细胞活性,作为考核药物抗病毒作用.结果表明:大青叶有效单体1#,2#,3#,4#对CVB3无直接灭活作用,也不能阻止CVB3的吸附,而能抑制CVB3在Hep-2细胞内的的生物合成.其半数抑制浓度(IC50)分别为30.75,28.54,35.31,26.15 mg/L,治疗指数(TI)分别为6.51,9.71,8.24,14.96.其抗CVB3效果优于病毒唑(IC50=103.13 mg/L,TI=5.22),和抗病毒口服液(IC50=105.44 mg/L,TI=5.02),P>0.01.在2～128 mg/L范围内大青叶1#,2#,3#,4#单体与CVB3抑制率呈明显的量效关系(P<0.01),在128 mg/L时能抑制CVB3在Hep-2细胞内的增殖达90%左右.大青叶有效单体1#,2#,3#,4#能安全高效地抑制CVB3在Hep-2细胞中的增殖.The antiviral activity of the 4 effective monomers from Chinese medicinal herb Folium Isatidis （FI） No. 1,2,3 and 4 against Coxackievirus B3 virus was obtained by cell culture technigue and MTT colorimetric assay to observe cytopathic effect （CPE） and to get virus inhibitory rate. The results showed that the four monomers may inhibit CVB3 biological synthesis other than directly inactivate this virus or block its adsorption to susceptible cells. The inhibitory concentration （IC50） of the four monomers were 30. 75, 28. 54, 35. 31, 26. 15 mg/L, respectively. And the treatment index（TI） were 6. 51, 9. 71, 8. 24 and 14. 96 respectively. Their antiviral activity were more offective than ribavirin （IC50=103.13 mg/L, TI=5.22） and antiviral liquor （IC50 =105. 44 mg/L, TI =5.02） at the same dosage level （P〈0.01）. Moreover, there are obvious dosage-effect relationship between the 2 -128 mg/L dose of the 4 monomers and the anti-CVB3inhibitory rate in vitro （P〈0.01）. A concentration of 128 mg/L of the monomers can nearly inhibit 90 percents of the proliferation of CVB3 in Hep-2 cells. We conclude that the 4 monomers from Folium Isatidis can inhibit the profication of the CVB3 in Hep-2 cell effectively and safely.

关 键 词：大青叶 单体 柯萨奇病毒 抗病毒作用 

分 类 号：Q523.3[生物学—生物化学]