高频正弦波振动对制动大鼠比目鱼肌萎缩的对抗作用  

Countermeasure of Muscular Atrophy of Immobilization Rats by High Frequency Sinusoidal Vibration

作　　者：张德芳[1] 任俊婵[2] 

机构地区：[1]陕西能源职业技术学院护理系,陕西西安710613 [2]西安电子科技大学生物医学工程系,陕西西安710071

出　　处：《陕西能源职业技术学院学报》2009年第2期29-32,共4页

摘　　要：目的探讨高频正弦波振动对后肢制动大鼠比目鱼肌萎缩的对抗作用。方法采用大鼠后肢制动（石膏固定）作为肌肉废用模型，将成年SD大鼠按随机配对原则分为3组：对照组（CON）、制动组（IM）、制动＋高频振动组（IM＋HFV）。每组10只动物。在后肢制动的14天期间，对IM＋HFV组大鼠的制动肢体的比目鱼肌部位实施高频振动（100Hz；500μm）。14天后，取大鼠比目鱼肌称其湿重，计算出湿重体重比；采用酶组化染色及计算机图像分析技术测定骨骼肌纤维的横截面积以及I、II型肌纤维的构成比。结果1）14天后，制动组大鼠比目鱼肌湿重、湿重／体重及肌纤维横截面积与对照组相比明显减小（P〈0．05）：同时，制动组大鼠比目鱼肌I型肌纤维所占比例下降而II型肌纤维比例增高（P〈0．05）。2）制动加高频振动组大鼠14天后，与制动组相比，比目鱼肌的湿重、湿重／体重及肌纤维横截面积均有明显增大（P〈0．05）。结论高频正弦波振动可有效地对抗制动引起的比目鱼肌萎缩。Objective To investigate whether high frequency sinusoidal vibration could partially or totally prevent soleus atrophy during 14 - day hindlimb immobilization. Methods Thirty mature female Sprague Dawley （ SD ） rats were randomly assigned to ambulatory control （Con）, hindlimb immobilized alone （IM） , and hindlimb immobilized with vibration （IM- V）. Throughout 14- day hindlimb immobilizated period, The vibrations （100Hz; 500μm） were applied to the immobilized soleus muscle in IM - V group. The soleus muscles were examined 14 days later. Sections were treated by using the modified method of Torrela JR et al. The cross - sectional areas （CSA） fibers, the fiber -type distribution were measured by Leica image analysis system. Results ① Compared with control , a significant decrease in the muscle wet weight （MWW） and the ratio of Wet weight - to - body weight（MWW/BW） ,and in the fiber CSAs of each kind of fiber type, as well as an evident shift toward a higher percentage of fast fibers （ type I： from 83.1% to 68.9% ; type II ： from 16.9% to 31.1% ）. （P 〈 0. 05）. ② Application of high frequency sinusoidal vibration during IM significantly counteracted the atrophic response by preventing 56% of the reduction in type I myofiber cross-sectional area （CSA） and preventing 61% of the reduction in type II myofiber CSA of the soleus, while preventing 71% , 79% of the decrease in MWW and MWW- to -BW ratio of soleus, respectively. But high frequency sinusoidal vibration during IM induced no change in fiber - type distribution, compared with hindlimb - immobilizing values （ P 〉 0. 05 ） . Conclusions High frequency sinusoidal vibration applied to the soleus muscle can be used as an effective countermeasure to muscle atrophy induced by hindlimb immobilizing.

关 键 词：后肢制动 肌肉萎缩 对抗措施 高频正弦波振动 

分 类 号：R852[医药卫生—航空、航天与航海医学]