油酸诱导的急性肺损伤模型肺泡液体清除能力的实验研究  

The study of alveolar fluid clearance in oleic acid-induced acute lung injury of rats

作　　者：周望梅[1] 陈东升[1] 桑显富[1] 鲍光欣[1] 于纯文[1] 何琳[1] 

机构地区：[1]南方医科大学南方医院急诊科,广州510515

出　　处：《广东医学》2009年第7期1021-1023,共3页Guangdong Medical Journal

基　　金：广东省医学科研基金项目(编号:A2008404)

摘　　要：目的探讨油酸诱导的急性肺损伤模型肺泡内液体的清除能力。方法清洁级雄性大鼠51只随机分为正常组、急性肺损伤组和特布他林治疗组。油酸静脉注射复制急性肺损伤模型。动脉血气、肺组织病理学检测评估肺损伤程度,电镜下观察急性分离的肺泡Ⅱ型上皮细胞的变化,重力法测定血管外肺水量(EVLWI)。结果急性肺损伤组大鼠肺损伤评分为(6.37±1.26)分、血管外肺水量为(4.02±0.69)mL/g,显著高于正常组[(1.39±0.5)分和(2.73±0.50)mL/g],均P<0.05;特布他林治疗后大鼠肺损伤评分为(5.23±1.14)分,血管外肺水量为(3.50±0.45)mL/g,较急性肺损伤组显著下降(均P<0.05)。电镜下观察急性肺损伤组肺泡Ⅱ型上皮细胞形状不规则,染色质边集,线粒体肿胀,板层小体排空及空泡化等;经特布他林治疗后,电镜观察大鼠肺泡Ⅱ型上皮细胞线粒体肿胀程度及板层小体排空程度较急性肺损伤组轻。结论油酸致急性肺损伤大鼠肺泡液体清除能力下降,特布他林可以减轻肺组织及肺泡Ⅱ型上皮细胞的损伤,提高肺水清除能力。Objective To determine the capability of alveolar fluid clearance in oleic acid - induced acute lung injury （ALl） in rats. Methods Fifty one male Sprague - Dawley rats were randomized into three groups： control group, ALl group and terbutaline treatment group. The degree of injury was determined by arterial blood gas and histopathologieal changes of the lungs. Cellular uhrastrueture was examined by transmission electron mierograph. The extravasular lung water （EVLW） content was quantitated by gravimetric method. Results In ALl group, Smith lung injury score （6.37 ± 1. 26） score and EVLWI [ （4.02 ±0. 69） mL/g] were significantly higher than those in the control group [ （ 1.39 ± 0.5 ） score and（ 2.73 ± 0.50 ）mL/g, P 〈 0.05 ]. Transmission electron microscopy showed irregular cell shape, chromatin margination,mitochondria tumefaetion, vaeuolated lamellar bodies in alveolar type ]/cells （ AT 11 ）. After terbutaline treatment, Smith lung injury score （ 5.23 ± 1.14 ） score and EVLWI [ （ 3.50 ± 0.45 ） mL/g ] were significantly lower than those of ALl group （ P 〈 0. 05 ）. Transmission electron microscopy showed that the degree of cellular damage was reduced. Conclusion The capacity of alveolar fluid clearance is impaired in oleic acid - induced acute lung injury. Terbutaline reduces lung and AT Ⅱ cells injury and improves alveolar fluid clearance.

关 键 词：急性肺损伤 大鼠 油酸 特布他林 急性呼吸窘迫征 

分 类 号：R563[医药卫生—呼吸系统] R541.630.2[医药卫生—内科学]