福辛普利对压力超负荷性心肌肥厚大鼠左心室心肌细胞凋亡的影响  

THE EFFECTS OF FOSINOPRIL ON APOPTOSIS OF MYOCARDIAL HYPERTROPHY RAT WITH PRESSURE OVERLOAD

作　　者：薛明明[1] 吴莉[2] 

机构地区：[1]内蒙古医学院基础医学院生理教研室,内蒙古呼和浩特100059 [2]内蒙古医学院附属医院麻醉科

出　　处：《内蒙古医学院学报》2009年第3期204-208,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金(200607010914)

摘　　要：目的:研究福辛普利(fosinopril)对压力超负荷性心肌肥厚大鼠左心室心肌细胞凋亡的影响。方法:将36只Wistar大鼠随机分为假手术组、腹主动脉缩窄组、腹主动脉缩窄+福辛普利给药组。以左心室重量指数作为心肌肥厚的指标;以DNA荧光染色方法测凋亡率研究心肌细胞凋亡的情况。后者采用流式细胞术测试。结果:与假手术组相比,腹主动脉缩窄组大鼠心肌细胞凋亡率明显升高(P<0.01);腹主动脉缩窄+福辛普利给药组同腹主动脉缩窄组相比,心肌细胞凋亡率下降(P<0.05)。结论:(1)心肌细胞凋亡是压力超负荷性心肌肥厚发生的一个重要因素;(2)福辛普利能有效地抑制心肌细胞凋亡,从而使心肌肥厚发生逆转。Objective： To investigate the effects of fosinopril on the myocardial apoptosis of myocardial hypertrophy rat with pressure overload. Methods：36 Wistar rats were randomly divided into three groups： false operation group, abdominal aorta constriction group and abdominal aorta constriction ＋ fosinopril treatment group. The ratio of left ventricular weight vs body weight（LVW/BW） was regarded as the index of myocardial hypertrophy; the DNA fluorescent stain method was used to show the incidence of myocardial apoptosis. The latter was tested by flow cytometry （FCM）. Results： Comparing with false operation group, the myocardial apoptosis of abdominal aorta constriction group was increased significantly（P 〈0.01 ）. The myocardial apeptosis of bax gene of abdominal aorta constriction ＋ fosinopril treatment group is lower significantly（ P 〈 0.05 ）. Conclusion： （ 1 ） Myocardial apeptosis is a important reason of myocardial hypertrophy with pressure overload ; （2） Fosinopril effectively inhibits myocardial apoptosis, which can contribute to its role of reducing myocardial apoptosis.

关 键 词：心肌肥厚 细胞凋亡 福辛普利 

分 类 号：R331.3[医药卫生—人体生理学] R972.4[医药卫生—基础医学]