HPLC法测定清热解毒方芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量  

Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Rheochrysidin in QING RE JIE DU FANG by HPLC

作　　者：薛小平[1] 鹿燕敏[1] 王倩[1] 刘洪斌[1] 

机构地区：[1]天津市南开医院中西医结合急腹症研究所,天津300100

出　　处：《中国实验方剂学杂志》2009年第7期6-8,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市科技攻关计划重大科技项目(05YFGDSFO2600)

摘　　要：目的:建立反相高效液相色谱法测定清热解毒方中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含量的方法。方法:采用HPLC法,色谱柱:Kromasil C18(250 mm×4.6 mm,5μm),以甲醇-0.2%磷酸(85∶15)为流动相,流速:1.0 mL.min-1,检测波长:254 nm,柱温:35℃。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在1.012 5～16.2μg、1.025～16.4μg、1.025～16.4μg、1.025～16.4μg、0.625～10μg范围内呈良好线性关系,相关系数分别为0.999 1,0.999 8,0.999 7,0.999 4,0.999 6,平均回收率分别为101.4%,102.0%,101.7%,102.1%,100.8%。结论:该方法操作简单、准确,且重复性好,可作为清热解毒方质量的控制指标之一。Objective： To develop an HPLC method for determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in QING RE JIE DU entraet. Methods： The analysis was carried out on a Kromasil C18 （250 mm × 4.6 mm, 5 μm）column eluted with a mobile phase of methanal-water containing 0.2% phosphoric acid solution（V：V = 85：15）. The flow rate was 1.0 mL·min^-1 and column temperature was 35℃. The detection wavelength was set at 254 nm. Results： The calibration curve was linear within the range of 1.012 5 - 16.2,1.025 - 16.4,1.025 - 16.4,1.025 - 16.4 and 0. 625 - 10μg for aloe-emodin, rhein, emodin, chrysophanol and physcion, respectively. The average recoveries were 101.5 % ,99.2 %, 101.9 %, 101.5 %, 101.4 % for the abore five conponents, respectively. Conclusion： This method is convenient, accurate and reproducible for determination of aloe-emodin, rhein, emodin, chrysophanol and phascion in QING RE JIE DU extraet.

关 键 词：反相高效液相色谱法 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 清热解毒方 

分 类 号：R284.1[医药卫生—中药学]