通脉新复方对系膜增殖性肾炎病理学改变的影响  

The Pathological Study on Effective Compounds of TongMai in Treating MsPGN in Rats

作　　者：刘明平[1] 黄兆胜[2] 杨霓芝 韦品清[1] 吴庆光[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学护理学院,广东广州510006 [3]广州中医药大学第二附属医院,广东广州510120

出　　处：《中国实验方剂学杂志》2009年第7期54-57,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30271605)

摘　　要：目的:观测通脉新复方对系膜增生性肾炎(MsPGN)病理学改变的影响。方法:采用抗胸腺抗血清(ATS)制备大鼠MsPGN肾炎模型及行疲劳游泳致气虚血瘀证模型,分别ig给药高、中、低剂量(1.2,0.6,0.3 g.kg-1)通脉新复方4周。4周后进行肾组织病理学检查、免疫荧光检测IgG以及免疫组化法测定肾小球内细胞核增殖抗原PCNA的表达。结果:通脉新复方能抑制肾小球系膜增生,降低系膜区基质占毛细血管丛面积的百分比,减少大鼠肾脏系膜区IgG沉积,显著降低肾小球内PCNA阳性细胞数。结论:通脉新复方能改善系膜增生性肾炎大鼠模型肾组织病理损伤。Objective： To observe the therapeutic effects of effective compounds which were extracted from TongMai. Methods： SD rats experimential model with MsPGN were induced by subcutaneously injection rabbit anti-rat thymocyte serum（ATS） and forced swim. The rats were respectively ig with different dose （1.2,0.6,0.3 g·kg^-1） of effective compound of TongMai for 4 weeks. Then the proliferative degree of mesangium was examined by HE stain, PAS stain and immunfluorescent respectively. Mesagium area proportion in glome＇rular capillaries area was calculated using image analyzing system. The expression of PCNA in the glomeruli was examined by immunohis to chemistry. Results ： The effective compounds of TongMai can control the hyperplasia of the glomerular mesangial cells （GMG）and the mainifold of the mesangial matrix, and prevent the deposit of IgG in mesangium. The proportions in glomerular capillaries area in model control group were significantly higher than that in the treatment groups; The express of proliferative cell nuclear antigen （PCNA） were ascended either. Conclusion： The effective compound of TongMai can improve the damage s of pathology in glomerular.

关 键 词：通脉新复方 系膜增生性.肾炎 病理损伤 

分 类 号：R285.5[医药卫生—中药学]