桑黄云芝胶囊对小鼠肉瘤S_(180)及肝癌H_(22)移植性肿瘤生长的抑制作用  

Anti-tumor Effect of Phellinus Linteus and Coriolus Versicolor Capsule on S_(180) sarcoma and H_(22) hepatoma in mice

作　　者：李坤星[1] 朱学萍[1] 张海林[1] 聂克[1] 郭炜[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2009年第7期83-86,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察桑黄云芝胶囊对小鼠肉瘤S180及肝癌H22实体瘤的抑瘤作用及对肝癌H22腹水型小鼠生存期的影响。方法:昆明小鼠分别右腋皮下接种肉瘤S180及肝癌H22瘤种,连续灌胃给药12 d后处死动物,剥离皮下肿瘤并称重。S180瘤块用4%多聚甲醛固定,用免疫组织化学法检测瘤组织中血管内皮生长因子(VEGF),CD4及CD8的表达。另取昆明小鼠ip肝癌H22瘤种,连续桑黄云芝胶囊ip 10 d,停药后继续观察至小鼠死亡,记录小鼠生存天数,计算生命延长率。结果:与模型组相比,桑黄云芝胶囊可使S180和H22瘤重明显减轻,抑制VEGF表达,增强CD4和CD8表达,可明显延长H22肝癌腹水型荷瘤小鼠的生存天数。结论:桑黄云芝胶囊可抑制肿瘤生长,延长荷瘤小鼠生存期,其作用机理部分与抑制新生血管生成、增强机体免疫力有关。Objective： To observe the anti-tumor effect of Phellinus Linteus and Coriolus Versicolor Capsules （PLCVC） in S180 sarcoma and H22 hepatoma animal models in mice. Methods： The sarcoma S180 and hepatoma H22 models were established in mice. After 12 days of treatment, the animals were killed, and the subcutaneous sarcoma were separated and weighted. The levels of vascular endothelial growth factor（VEGF）, CD4 and CD8 of S180 tumor tissue were investigated by immunohistochemical method. KM mice were intraperitoneal injected with H22 hepatoma cells, and treated with different experimental drugs. The survival time was observed and recorded, and life-prolongation rate was calculated. Result： PLCVC could inhibit the growth of S180 and H22 tumor, and inhibit the expression of VEGF, improve the expression of CD4 and CD8. The survival time of the group. Conclusion： PLCVC can inhibit the growth of and improving the immunological function mice treated by PLCVC were significantly longer than the untreated tumour, the mechanism is partially related to inhibiting angiogenesis

关 键 词：桑黄云芝胶囊 肉瘤S180 肝癌H22 血管内皮生长因子(VEGF) 生存期 

分 类 号：R285.5[医药卫生—中药学]