黄芩苷、连翘酯苷对肺微血管内皮细胞分泌IFN-α、IFN-γ的影响  

Effect of Baicalin and Forsythiaside on the Excretion of IFN-α and IFN-γ in Pulmonary Microvascular Endothelial Cells

作　　者：林红[1] 胡格[1] 伊鹏霏[2] 蒋小林[1] 穆祥[3] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]吉林大学畜牧兽医学院,长春130062 [3]北京农学院兽医学(中医药)北京市重点实验室,北京102206

出　　处：《中国畜牧兽医》2009年第6期30-34,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30500368);北京市自然科学基金A类重点项目(6061001);北京市科技新星项目(2006A26);北京市教委"学术创新团队"(5090245)

摘　　要：为了研究黄芩苷、连翘酯苷对大鼠肺微血管内皮细胞(pul monary microvascular endothelial cells,PMVECs)分泌IFN-α、IFN-γ的影响,本试验以体外培养的PMVECs为模型,用ELISA法检测这2种中药成分在不同浓度、不同时间点诱导PMVECs分泌IFN-α和IFN-γ的量。结果表明,黄芩苷在10μg/mL,24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量均最高,连翘酯苷分别在20μg/mL、12 h时和5μg/mL、24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量最高。这一结果为体外筛选诱导PMVECs表达干扰素的中药成分提供了参考,为进一步研究中药成分的抗病毒机理奠定了基础。To study the effect of baicalin and forsythiaside to induce rat pulmonary microvascular endothelial cells to ex- press IFN-α and IFN-γ, the model was based on cultured PMVECs in vitro. With the method of ELISA, we detected the secretory volume of IFN-α and IFN-7 after PMVECs was induced by baiealin and forsythiaside at different concentration and time. The results indicated that PMVECs produced the most IFN-α and IFN-γ after induced 24 h by baicalin at 10 μg/mL, while forsythiaside was 20 μg/mL at 12 h and 5 μg/mL at 24 h, respectively. The result provided references for screening Chi- nese herbal medicinal constituents that could induce PMVECs to express IFN, and laid the foundation to further study the anti- virus mechanism of medicinal constituents.

关 键 词：黄芩苷 连翘酯苷 肺微血管内皮细胞 IFN-Α IFN-Γ 

分 类 号：S853.74[农业科学—临床兽医学]