不同猪种ESR基因多态性的PCR-RFLP分析  

Polymorphisms of Porcine ESR Gene in Different Breeds with PCR-RFLP Method

作　　者：曹晶晶[1] 蔡家嘉[1] 黄小国[2] 何晓亮[2] 包文斌[1] 吴圣龙[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]常州市康乐农牧有限公司,常州213149

出　　处：《中国畜牧兽医》2009年第6期82-84,共3页China Animal Husbandry & Veterinary Medicine

基　　金：常州市农业科技攻关项目(BG2006302);江苏省高校自然科学基础研究项目(08KJB230004)

摘　　要：运用PCR-RFLP方法,检测了杜洛克、约克夏、长白猪、皮特兰、梅山猪、二花脸猪、枫泾猪、荣昌猪、苏太猪等9个国内外猪种的ESR基因PvuⅡ位点多态性。结果表明,在约克夏猪、二花脸猪、枫泾猪和苏太猪中检测到了AA、AB、BB 3种基因型,在杜洛克猪、皮特兰猪中检测到AB、BB 2种基因型,在长白猪、梅山猪、荣昌猪中只检测到了AB基因型。在本研究中不同品种的A、B基因频率差异显著,总体上在国外猪群体中A等位基因为优势等位基因,而地方猪种中B等位基因为优势等位基因。Pvu Ⅱ polymorphisms of ESR gene were analyzed in nine native and exotic pig breeds including Duroc, York- shire, Landrace, Pietrain, Meishan, Erhualian, Fengjing, Rongchang and Sutai pigs with PCR-RFLP method. The results showed that AA, AB, BB genotypes were detected in Yorkshire pigs, Erhualian pigs, Fengjing pigs and Sutai pigs, while only AB and BB genotypes were found in Duroc pigs and Pietrain pigs, only AB genotypes were detected in Landrace pigs, Meishan pigs and Rongchang pigs. There was a significant difference in gene frequencies and genotype distribution among different breeds. The allele A was dominant allele ,in domestic pig breeds while the allele B was dominant allele in native pig breeds.

关 键 词：猪 PCR-RFLP ESR基因 多态性 

分 类 号：Q786[生物学—分子生物学]