猪干扰素-γ基因单核苷酸多态性分析  

Single Nucleotide Polymorphism Analysis of IFN-γ Gene in Pigs

作　　者：王大力[1,2] 刘畅[1] 王秀娜[2] 黄大鹏[2] 孙博兴[1] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《中国畜牧兽医》2009年第6期90-94,共5页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省科技发展计划项目(20080208)

摘　　要：干扰素-γ(interferon-gamma,IFN-γ)在调节机体免疫应答及抵抗病毒感染等方面有着重要的作用。为探索IFN-γ基因作为猪抗病育种中一种分子标记的可能性,采用PCR-SSCP及测序方法对包括军牧1号白猪、杜洛克猪、约克夏猪3个品种共481个个体的IFN-γ基因全序列的单核苷酸多态性进行分析。结果在猪IFN-γ基因的内含子1、内含子3、外显子4上各发现了一个突变位点,分别为T825C、T2730C、G5301A。除在约克夏猪中没有检测到G5301A突变外,各遗传群体内T825C、T2730C、G5301A位点均处于Hardy-Weinberg平衡状态。各个突变位点的多态信息含量均小于0.25,呈低度多态,说明猪IFN-γ基因较保守。Interferon-gamma （IFN-γ） plays an important role in the innate host defense against viral infection. In order to analyze the value that the IFN-γ gene act as a genetic marker in porcine disease resistance, single nucleotide polymorphisms （SNPs） in the whole region of the IFN-γ gene were studied by use of PCR-SSCP approach in 481 pigs from the ＂Junmu No. 1＂ White, Landrace, Duroc pigs. Three SNP sites were detected： T825C in Intron 1, T2730C in Intron 3 and G5301A in Extron 4. Except for the absence of G5301A in Duroc pigs, all the other sites in the all populations were in a state of Hardy-Weinherg equlibrium. The PIC of every sites had low polymorphisms（〈0.25）, which represented the own unique conservativeness of IFN-γ gene in pigs.

关 键 词：猪 干扰素-Γ 单核苷酸多态性 

分 类 号：Q754[生物学—分子生物学]