稀释方法对绒山羊冷冻精液品质的影响  

Effects of Dilution Methods on Frozen Semen Quality of Cashmere Goats

作　　者：路佩瑶[1] 任有蛇[1] 申慧敏[1] 岳文斌[1] 姚继广 杨子森 王慧生 赵兴财 白海全 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]山西省生态畜牧产业管理站,太原030001 [3]岢岚县畜牧兽医局,岢岚036300 [4]岢岚县绒山羊种羊场,岢岚036300

出　　处：《中国畜牧兽医》2009年第6期101-103,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家“十一五”科技支撑计划项目(2007BAD56B03);山西省青年科学基金项目(20041035)资助

摘　　要：选取7只精液品质优良的雁门绒山羊种公羊,通过测定其冷冻精液解冻后的精子活率(0、4 h)、精子存活指数、顶体完整率及精清中透明质酸酶(HYD)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)酶活力,研究一步稀释法和二步稀释法对冷冻精液品质的影响。结果表明,二步稀释法解冻后的精子存活指数显著(P<0.05)高于一步稀释法,其解冻后精清中透明质酸酶、谷草转氨酶、乳酸脱氢酶的活力均显著(P<0.05)低于一步稀释法。表明采用二步稀释法冷冻的绒山羊精液品质好于一步稀释法。Seven cashmere goats producing great semen was selected. Sperm motility（0, 4 h）, sperm survival index, acro- somal integrity, enzyme activity of HYD, GOT and LDH after frozen-thawed were measured, to study the effect of one-step dilution method and two-step dilution method on semen freezing and frozen-thawed spermatozoa quality. The results showed that sperm survival index of two-step dilution method was higer significantly than that of one-step dilution method after frozen- thawed. Enzyme activity of HYD, GOT and LDH of two-step dilution method was lower significantly than those of one-step. The results showed that frozen semen quality of cashmere goats of two-step dilution method was better than that of one-step di lution method.

关 键 词：绒山羊 稀释方法 冷冻精液 

分 类 号：S814.4[农业科学—畜牧学]