奶牛隐性乳房炎的病原检测与小白鼠致病性研究  

Study on the Detection of Pathogen and Pathogenicity on Mouse about Dairy Cow Subclinical Mastitis in Xinjiang

作　　者：张雪梅[1] 刘明军[1] 杨波[2] 谭立新[1] 张宁[1] 玛依拉[1] 

机构地区：[1]新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,乌鲁木齐830000 [2]新疆农业大学,乌鲁木齐830052

出　　处：《中国畜牧兽医》2009年第6期119-123,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家973前期专项(2007CB116304)

摘　　要：本研究以新疆乌鲁木齐地区的2个奶牛场隐性乳房炎患牛为试验对象,共分离鉴定出21种91株引起奶牛乳房炎的主要病原菌,其中葡萄球菌28株(30.78%),链球菌21株(23.07%),杆菌25株(27.48%),其他细菌17株(18.67%)。对主要病原菌通过动物试验确定致病力较强的菌为金黄色葡萄球菌、克雷伯氏菌和大肠杆菌。同时对17大类主要病原菌共30种药物的药敏试验结果表明,在奶牛隐性乳房炎中应用较多的青霉素、四环素等抗生素对常见致病菌几乎无效,而左氧氟沙星、呋喃妥因、头孢噻肟、阿米卡星则显示出较好的抑菌作用。Milk samples of subclinical mastitis were collected from two dairy cow farms in Xinjiang, 91 strains of bacteria which contain 21 species were isolated and identificated from milk specimens. There were 28 strains of Staphylococci, 21 strains of Streptococcus, 25 strains of bacillus and 17 other strains, the rate was 30.78%, 23.07%, 27.48% and 18.67% re- spectively. Animal test of the main pathogenic bacteria had certained that the higher pathogenicity strains were Staphylococcus aureus, Klebsiella and E. coll. The results of 30 drugs sensitivity test showed pathogenic bacteria almost were ineffective to penicillin and tetracycline frequently used, while levofloxacin, furadantin, cefotaxim and amikacin had a fairly good bacteriostasis.

关 键 词：奶牛隐性乳房炎 病原菌 分离鉴定 致病性 药敏试验 

分 类 号：S857.26[农业科学—临床兽医学]