冷冻家猫附睾精液效果初探  

Effect of Cryopreservation on Domestic Cat Epididymal Spermatozoa

作　　者：徐杨[1] 鲍文玉[1] 郭勇[1] 邬静[1,2] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]西北农林科技大学动物医学院,杨凌712100

出　　处：《中国畜牧兽医》2009年第6期210-213,共4页China Animal Husbandry & Veterinary Medicine

基　　金：北京市教育委员会科技发展计划面上项目资助(KM200710020006);2007年度教育部留学人员科技活动择优资助项目(启动类)部分资助

摘　　要：为了筛选出适宜冷冻家猫精液的稀释液、冷冻保护剂和解冻温度,本试验采用家猫附睾精液,分别用2种不同的稀释液,3种不同的冷冻保护剂进行稀释、冷冻和解冻。结果显示,Ⅱ号稀释液冷冻效果优于Ⅰ号稀释液。用乙二醇作为冷冻保护剂,解冻后家猫精子活率达到52.7%±4.9%,畸形率为37.3%±4.2%,顶体完整率为52.4%±4.1%,各项指标均显著高于甘油组和二甲基亚砜组(P<0.05);其中甘油组解冻后活率为35.5%±7.6%,顶体完整率为39.8%±4.4%,高于二甲基亚砜组的33.6%±7.3%和39.1%±4.1%,但两者之间差异不显著(P>0.05)。37℃水浴解冻后顶体完整率为36.4%±8.7%,显著高于30℃水浴解冻后的25.3%±6.7%(P<0.05),但它们之间的活力,畸形率差异不显著,总体上37℃的解冻效果优于30℃。因此,乙二醇和稀释液Ⅱ配合使用冷冻家猫附睾精液效果较好,37℃为较优解冻温度。To search the better extender, cryoprotectant and thaw temperature for cryopreservate domestic cat epididymal spermatozoa, the domestic cat epididymal spermatozoa was collected and assessed before and after rapid cryopreservation by two different extenders and three different cryoprotectants respectively. Then, they were thawed in warm water bath either at 37 22 or 30 22. The results showed that extender II was much more effective than another one. As a cryoprotectant, the glycol was also much better than the glycerol and DMSO（P〈0.05）. The post-thawing motility and abnormal sperm rate of the frozen/thawed spermatozoa protected by glycol reached 52.7±4.9 % and 37.3 %±4.2% respectively. Its intact acrosome rate was 52.4 % ±4.1%, but there was no significant difference between the group with glycerol and that with DMSO. It indicated that the glycol and extender Ⅱ was better for eryopreservate domestic cat epididymal spermatozoa, thawing effect at 37 ℃ was much better than that at 30℃.

关 键 词：家猫 附睾精液 冷冻 

分 类 号：S829.3[农业科学—畜牧学]