标本溶血对SD大鼠14项生化检测结果的影响与分析  

Influence of Hemolysis on Fourteen Biochemical Items in SD Rat Blood

作　　者：王振宇[1] 宋京风[1] 陆瑛[1] 

机构地区：[1]昆明医学院云南省天然药物药理重点实验室,昆明650031

出　　处：《实验动物科学》2009年第3期18-20,共3页Laboratory Animal Science

摘　　要：目的探讨标本溶血对SD大鼠14项生化检测结果的影响。方法采用TMS-1024全自动生化分析仪对20只大鼠不同溶血程度即Hb含量为0、12、、35、、10g/L的血清样本进行14项生化指标检测,并对结果进行统计学分析。结果溶血引起ALT、AST、TP、LDH、CK、TBi、UREA检测结果升高(P<0.05/0.01);引起CR、TG、A/G检测结果的降低(P<0.05/0.01);对GLU、CHO、ALP、ALB检测结果的影响不明显(P>0.05)。结论样本溶血可对较多的生化指标产生明显的影响,并且随溶血程度的增加,产生的影响越明显,其中以LDH、TG和AST最为敏感。Objective To investigate the influence of hemolysis on 14 biochemical items in SD rat blood. Methods Blood samples were collected from 20 SD rats, and using the TMS-1024 auto-biochemical analyzer, fourteen serum biochemical items were detected from each sample based on the different Hb contents, 0, 1, 2, 3, 5, and 10 g/L. Results Hemolysis resulted in increasing of ALT, AST, TP, LDH, CK, TBi, and UREA levels （ P 〈 0.05/0.01） and decreasing of CR, TG, and A/G levels （P 〈 0.05/0.01） in sera. But the levels of GLU,CHO, ALP, and ALB were not significantly affected by hemolysis （ P 〉 0.05）. Conclusion A lot of biochemical items were significantly affected by hemolysis, and the levels of LDH, TG and AST are the more severely affected than others.

关 键 词：SD大鼠 血清 溶血 生化检测 

分 类 号：R446.1[医药卫生—诊断学] Q95-33[医药卫生—临床医学]