医院分离艰难梭菌耐药性研究  

Study on drug resistance of Clostridium difficile isolated from hospital

作　　者：程颖[1] 卢金星[1] 鄢盛恺[2] 贾红兵[2] 李文革[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206 [2]卫生部中日友好医院检验科

出　　处：《疾病监测》2009年第5期346-348,共3页Disease Surveillance

基　　金：卫生部传染病重大专项资助项目(No.2008ZX10004-012)

摘　　要：目的通过对临床分离的8株产毒型艰难梭菌进行PCR分型以及抗生素敏感性分析,初步了解临床分离艰难梭菌的耐药情况,为临床用药提供参考依据。方法临床送检的粪便标本,经生化鉴定和毒素检测后,确定8株为产毒型艰难梭菌,分别采用PCR方法进行分型,并采用Etest方法测定8株艰难梭菌对氨比西林(AC)、克林霉素(CM)、甲硝唑(MZ)、万古霉素(VA)的最小抑菌浓度,并对耐药性进行初步分析。结果8株产毒型艰难梭菌中有5株属于同一型别,7株出现耐药;3株对AC耐药;7株对CM耐药;1株对MZ耐药;未检测到对VA耐药菌株。结论临床分离艰难梭菌耐药比例高,且存在多重耐药;临床应加强艰难梭菌及厌氧菌抗生素敏感性检测。Objective To investigate drug resistance of Clostridium difficile isolated from hospital by PCR ribotyping and drug resistance detection to 8 clinically isolated strains of Clostridium difficile which were toxin producing. Methods Eight swains of toxin producing Clostridium difficile were isolated from clinical stool samples by biochemical identification and toxin detection, then the PCR ribotyping and the detection of minimum inhibition concentration （MIC） of ampiciline, clindamycin, metronidazole, vancomycin by Etest to the strains were conducted respectively, and the drug resistance was analyzed. Results Of 8 strains toxin producing Clostridium difficile, 5 belonged to a ribotype and 7 showed drug resistance, i. e. 3 were resistant to ampiciline, 7 were resistant to clindamycin, 1 was resistant to metronidazole, no strain was detected to be resistant to vancomycin. Conclusion The drug resistance rate of Clostridium difficile isolated in hospital was high, and multi-drug resistance was observed. The drug susceptibility test of Clostridium difficile and other anaerobic bacterium should be strengthened in hospital.

关 键 词：艰难梭菌 ETEST 最小抑菌浓度 PCR分型 

分 类 号：R446.5[医药卫生—诊断学]