高渗氯化钠羟乙基淀粉40注射液高容量血液稀释对大鼠肝脏缺血再灌注损伤的影响  

Effects of hypervolemic hemodilution with hypertonic saline plus hetastarch solution 40 injectio on hepatic ischemia-reperfusion injury in rats

作　　者：杨泽勇[1] 吕浩[1] 杨立群[1] 俞卫锋[1] 孙玉明[1] 

机构地区：[1]第二军医大学东方肝胆外科医院麻醉科,上海市200438

出　　处：《中华麻醉学杂志》2009年第6期513-515,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30600584）

摘　　要：目的探讨高渗氯化钠羟乙基淀粉40注射液高容量血液稀释对大鼠肝脏缺血再灌注损伤的影响。方法雄性Wistar大鼠30只，体重300～350g，随机分为3组（n=10）：假手术组（S组）、缺血再灌注组（IR组）和高容量血液稀释组（HH组）。S组仅开腹，不阻断血管；IR组阻断肝门静脉和左肝动脉30min，再灌注2h；HH组30min内经尾静脉输注高渗氯化钠羟乙基淀粉40注射液10ml／kg进行高容量血液稀释，输注完毕后15min，行肝脏缺血再灌注。再灌注2h时，下腔静脉取血样，测定血清谷丙转氨酶（ALT）和谷草转氨酶（AST）的活性；取左肝叶组织，光镜下观察病理学结果，采用比色法测定丙二醛（MDA）含量，采用黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性。结果与S组比较，IR组和HH组血清ALT和AST的活性、肝组织MDA含量升高，肝组织SOD活性降低（P〈0．01），肝组织病理学损伤明显；与IR组比较，HH组血清ALT和AST的活性、肝组织MDA含量降低，肝组织SOD活性升高（P〈0．01），肝组织病理学损伤减轻。结论高渗氯化钠羟乙基淀粉40注射液高容量血液稀释可减轻大鼠肝脏缺血再灌注损伤，可能与氧自由基生成减少有关。Objective To investigate the effects of hypervolemic hemodilution （HH） with hypertonic saline plus hetastarch solution 40 injectio on hepatic isehemia-reperfusion （I/R） injury in rats. Methods Thirty male Wistar rats weighing 300-350 g were randomly divided into 3 groups （ n = 10 each） ： group Ⅰ sham operation （group S） ; group Ⅱ I/R and group Ⅲ HH. Partial liver ischemia was produced by clamping hepatic portal vein and left arteria hepatica for 30 rain with atraumatic mini-clamp, followed by 2 h of reperfusion in I/R group and HH group. In HH group the animals were infused hypertonie saline plus hetastarch solution 40 injectio 10 ml/kg through vena caudalis over 30 min and then hepatic I/R was performed 15 min after the infusion. The animals were killed at 2 h of reperfusion. The left liver was removed and blood sample was taken from inferior caval vein for determination of （1） seruin alanine amino transferase （ALT） and aspartate anfino transferase （AST） activities; （2） superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in the liver; （3） microscopic examination. Results The serum ALT and AST activities and MDA content in the liver were significantly higher, SOD activity in the liver significantly lower after hepatic I/R and pathological changes in the liver severer in group I/R and HH than in group S. The scrmn ALT and AST activities and MDA content in the liver were significantly lower, SOD activity in the liver significantly higher after hepatic I/R and pathological changes in the liver milder in group HH than in group I/R. Conclusion Hypervolemic hemodilution with hypertonic saline plus hetastarch solution 40 injectio can ameliorate hepatic I/R injury by decreasing oxygen free radical production in rats.

关 键 词：盐水 高渗 羟乙基淀粉 血液稀释 再灌注损伤 肝 

分 类 号：R614[医药卫生—麻醉学]