利用宏基因组数据筛选高产核黄素的基因片段  

Selection of high-producing riboflavin genes by using metagenomic data

作　　者：赵越[1] 王晖[1] 鲍楠[1] 池村淑道 李岭[1] 

机构地区：[1]东北大学中荷生物医学与信息工程学院,沈阳110003 [2]日本长浜生物科学与技术大学生命科学部

出　　处：《黑龙江大学自然科学学报》2009年第3期380-384,共5页Journal of Natural Science of Heilongjiang University

基　　金：2008年国家大学生创新实验计划支持项目

摘　　要：从基于网络平台的宏基因组数据库入手,通过使用ARSA查询功能确定具有高产核黄素功能蛋白质,并利用BLAST、Orf Finder以及FASTA程序对其相关序列进行筛选和比对,得到一些具有潜在应用价值的基因片段。通过进一步确定这些替代基因的物种来源和物缘关系,筛查出在马尾藻海、巴拿马运河以及加拉帕格斯群岛附近海域存在的一些海洋螺菌属和聚球藻属的微生物。这些微生物的基因组中存在具有高产核黄素能力的基因片段,可望为改进国内现有的核黄素生产技术提供线索。Starting from web - based metagenomic database, the inquiry function of the ARSA was used for identifying particular proteins with the capacity of producing riboflavin in large amount. The corresponding DNA sequences was screened and compared using the BLAST program, Orf - Finder and FASTA, and a number of genes with potential functions were retrieved. Thereafter, based on the inferred living environment and phylogenetic relationship, several species belonging to oceanospirillaceae and synechococcus microorganisms were identified, which exist in the Sargasso Sea, Panama Canal, and Galapagos Islands. These microorganisms have their genome containing gene sequences capable of overproducing riboflavin. The results may provide important clues for the improvement of riboflavin production.

关 键 词：宏基因组 核黄素 rib操纵子 基因 筛选 

分 类 号：Q563.2[生物学—生物化学]