不同给药途径及剂量的LNNA对小鼠血清一氧化氮及一氧化氮合酶水平的影响  

Effects of LNNA with different ways on NOS activity and NO contents in serum of mice

作　　者：施溯筠[1] 张善玉[1] 

机构地区：[1]延边大学药学院,吉林延吉133000

出　　处：《延边大学医学学报》2009年第2期96-98,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]探讨不同给药途径及剂量的一氧化氮合酶抑制剂N-硝基-L-精氨酸(LNNA)对小鼠血清一氧化氮合酶活性和一氧化氮浓度的影响.[方法]选择健康昆明种小鼠180只,采用腹腔注射、皮下注射和灌胃给药途径分别给予0.5,5.0,10.0,20.0,50.0 mg/kg LNNA,14 d后取血清测定一氧化氮合酶活性和一氧化氮浓度.[结果]外源性LNNA可抑制并降低小鼠血清一氧化氮合酶活性及一氧化氮浓度,其中以腹腔注射给予10.0 mg/kg LNNA抑制效果最为明显.[结论]外源性一氧化氮合酶抑制剂可抑制小鼠血清一氧化氮合酶的活性,从而降低血清一氧化氮浓度.OBJECTIVE To explore the effects of LNNA with different ways on NOS activity and NO contents in serum of mice. METHODS A total of 180 healthy mice were randomly divided into 18 groups with 10 mice in each group. The drug groups were giving with LNNA 0.5, 5.0, 10.0, 20.0, 50.0 mg/kg by intraperitoneal injection, hypodermic injection and intragastric administration. After 14 days, the activity of NOS and the contents of NO were detected in blood serum in different groups. RESULTS The activity of NOS and the contents of NO with giving LNNA groups in blood serum of mice were lower than those in normal groups, especially used by 10.0 mg/kg LNNA was lowest. CONCLUSIONS LNNA can decreases the activity of NOS to decrease the content of NO in theserum of mice.

关 键 词：硝基精氨酸 一氧化氮 一氧化氮合酶 小鼠 

分 类 号：R94[医药卫生—药剂学]