桑叶乙酸乙酯萃取物化学成分分析  

Study on chemical constituents of extract of acetic ether from the mulberry leaf

作　　者：姜玉兰[1] 李仁[2] 朴惠善[3] 

机构地区：[1]延边大学基础医学院医学美容教研室 [2]延边大学附属医院医技科室 [3]延边大学药学院药物化学教研部,吉林延吉133000

出　　处：《延边大学医学学报》2009年第2期99-101,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]提取分离桑叶中的化学成分,并对其进行结构鉴定.[方法]利用800 mL/L乙醇对桑叶进行提取,溶剂梯度萃取,硅胶柱层析分离,利用核磁共振氢谱及碳谱等波谱学方法确定各成分结构.[结果]从乙醇提取物的乙酸乙酯萃取部分得6种化合物,经结构鉴定分别为β-谷甾醇(Ⅰ)、胡萝卜苷(Ⅱ)、山奈酚-3-O--βD-葡萄糖苷(Ⅲ)、槲皮素-3-O-β-D-葡萄糖苷(Ⅳ)、槲皮苷(Ⅴ)及桑黄素-3-O--βD-吡喃葡萄糖苷(Ⅵ),其中化合物Ⅵ为首次从桑叶中分离得到.OBJECTIVE To study the chemical constituents extracted from the mulberry leaf and identify its structure. METHODS The compounds were extracted with 800 mL/L ethyl alcohol, isolated by the siliga column chromatography and identified by spectrocopic methods. RESULTS Six flavonoids were isolated from the mulberry leaf and their structures were identified as b-sitosterol （ Ⅰ ）, daucosterol （ Ⅱ ） kaempferol-3-O-β-D-glucopyranoside （ Ⅲ ）, quercetin-3-O-β-D-glucopyranoside （ Ⅲ ）, quereitrin （ Ⅴ ） morin-3-O-β-D-glucopyranoside （Ⅵ）. Compound Ⅵ is obtained from the mulberry leaf for first time.

关 键 词：桑叶 黄酮类 萃取和加工工业 

分 类 号：R284.2[医药卫生—中药学]