流式细胞术免疫分型在形态学诊断困难的白血病中的应用  

Application of Immunophenotyping with Flow Cytometry in 11 Patients with Leukemia Difficultly Diagnosed by Morphotyping

作　　者：冀虎岗[1] 张子康[2] 

机构地区：[1]内蒙古自治区医院检验科,内蒙古呼和浩特010017 [2]呼和浩特市第一医院检验科,内蒙古呼和浩特010020

出　　处：《内蒙古医学杂志》2009年第5期565-567,共3页Inner Mongolia Medical Journal

摘　　要：目的:应用流式细胞仪对11例临床形态学诊断困难的急慢性白血病进行快速、有效地免疫分型,协助临床诊断,以指导临床化疗。方法:采用单克隆抗体三色(FITC、PE、Per-CP)直接免疫荧光标记法及多参数流式细胞术(FCM)检测,分型根据抗体积分系统,并与FAB分型进行比较。结果:①2例临床形态学拟诊断毛细胞白血病,经FCM免疫分型表达为B淋巴细胞性白血病,得以确诊;1例慢性淋巴细胞白血病经FCM免疫分型诊为T淋巴细胞性白血病;②4例临床诊断急性淋巴细胞白血病(L2、L3)的患者经FCM免疫分型确诊为B淋巴细胞性白血病;③3例临床形态学诊断困难急性非淋巴细胞性白血病经FCM免疫分型确诊3例为髓系白血病。结论:流式细胞仪多参数白血病免疫表型分析,可以弥补FAB分型的不足,为临床诊断提供重要依据,并为白血病治疗措施的个体化提供依据。Objective： To study the immunophenotyping with flow cytometry rapidly and efficiently in 11 patients with acute or chronic leukemia. Methods： Immunophenotyping was performed by threelabeling with different antibodies conjugated with FITC, PE and Per- CP and analyzed with multiparameter flow cytometry for 11 patients with acute or chronic leukemia, which were difficult to be diagnosed clinically by morphology. Results： ① 2 patients clinically diagnosing as Hairy Cell Leukemia （HCL） were immunophenoyped as B lymphocytic leukemia by FCM; one patient clinically diagnosed as chronic lymphocytic leukemia were immunophenotyped as T lymphocytic leukemia. ②Four patients clinically diagnosed as acute lymphocytic leukemia （L2, L3） were immunophenotyped as B lymphocytic leukemia by FCM. ③ Three patients clinically diagnosed as acute nonlymphocytic leukemia were immunophenotyped by FCM as myeloid leukemia. Conclusion： Immunophenotyping by FCM may retrieve the faults of FAB phenotyping, providing important evidences for clinical diagnosis and guiding treatment individually.

关 键 词：白血病 免疫分型 流式细胞仪 

分 类 号：R446[医药卫生—诊断学] R557[医药卫生—临床医学]