花生种子发育过程中脂肪酸累积模式研究  

Fatty acid accumulation pattern in developing seeds of peanut

作　　者：李晓丹[1] 曹应龙[1] 胡亚平[1] 肖玲[1] 武玉花[1] 吴刚[1] 卢长明[1] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《中国油料作物学报》2009年第2期157-162,172,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(30471099);国家863计划(2006AA10A114)

摘　　要：通过对花生种子脂肪酸累积模式的研究,揭示花生脂肪酸组成的形成规律。研究结果显示,下针后10d内的花生种子可检测到9种脂肪酸,C16∶1和C18∶3在发育过程中由高到低,逐渐消失,其他7种脂肪酸含量则随着花生种子的发育逐渐积累增高。成熟的花生种子中油酸和亚油酸含量占脂肪酸总含量的81%左右。脂肪酸总量在接近成熟时有所下降。不同脂肪酸在累积过程中显示了高度相关性,C16∶1和C18∶3之间呈正相关,属于第一类;其余7种脂肪酸之间也均呈正相关,属于第二类。第一和第二类脂肪酸之间呈负相关。主成份分析表明,9种脂肪酸可以分成三类:C18∶1与C18∶2各为一类,其他7种脂肪酸为另一类,C18∶1与C18∶2之比是花生脂肪酸组成的决定性因素。综合分析表明,决定花生脂肪酸组成的关键基因主要是脂肪酸去饱和酶FAD2基因和SAD基因,有目的地对这两个基因的表达进行调控对于花生脂肪酸组成的改良有着重要的价值和意义。Fatty acid composition is the key factor to determine the quality of nutrition and storage of peanut oil. The aim of this paper is to study the accumulation pattern of fatty acids in peanut seeds and to understand the mechanism underlying the fatty acid composition in peanut. Results showed ten days after pegging,9 fatty acids were detectable in the seeds. C16：1 and C18：3 reduced from high to low and finally disappeared;the other seven fatty acids increased during seed development. Oleic acid and linoleic acid accounted for 81 percent of the total fatty acids in mature peanut seeds. High correlations were found between fatty acids in accumulation patterns C16：1 and C18：3 as a first group were positively correlated and the remaining seven fatty acids as the second group showed mutual positive correlations. The first and second groups of fatty acids were negatively correlated. Principal component analysis demonstrated that these nine fatty acids could be divided into three categories. The ratio of C18：1 and C18 ： 2 proved to be the key factor of peanut fatty acid composition. Total amount of the fatty acids dropped near maturity. SAD and FAD2 could be playing a decisive role in peanut fatty acid composition, and the controlling expression of two genes would have significant importance for peanut fatty acid improvement.

关 键 词：花生 种子发育 脂肪酸合成 相关分析 

分 类 号：S565.201[农业科学—作物学]